I-Research Express | Uphononongo lweYunivesithi yaseTsinghua lubonisa ukuba i-NMN inokunyanga ukudumba

Ukusebenza kweMacrophage yindlela ebangela izifo ekhokelela ekuvuvukeni okungapheliyo emzimbeni, kodwa ukusebenza kweMacrophage okungapheliyo kunokukhokelela ekuvuvukeni okungapheliyo kunye nezifo ezifana nokuxhathisa i-insulin kunye nezifo ezinzima ezifana ne-atherosclerosis. I-PGE 2, elawula impendulo yokuvuvuka, yenziwe kwi-arachidonic acid yi-cyclooxygenases (COX-1 kunye ne-COX-2). I-COX-1 kunye ne-COX-2 zezona zinto ziphambili ekujoliswe kuzo ekuvuvukeni kwaye zinokuthintelwa ngamayeza angengo-steroidal okuvuvukala (ii-NSAID).

Ukusetyenziswa kwe-NSAIDs kunokubangela iziphumo ezininzi ezingalunganga, ezinje ngokopha kwamathumbu. Ke ngoko, kubaluleke kakhulu ukufumana into yendalo ekhuselekileyo yokunyanga ukudumba.

Kutshanje, iqela lophando elivela kwiYunivesithi yaseTsinghua linyange ii-macrophages zempuku nge-NMN, laza labonisa ngokusebenzisa iimvavanyo ukuba i-NMN inokunciphisa ukuqokelelwa kweeproteni ezinxulumene nokuvuvukala kunye nemveliso ye-metabolic, kwaye ithintele impendulo yokuvuvukala kwee-macrophages. Imingcele kwiMolecular Biosciences.

Ukuvuvukala kutshintsha amanqanaba eemveliso ze-metabolic kwi-macrophages
Okokuqala, iqela lophando lisebenzise ii-macrophages ukuvelisa ukuvuvukala nge-lipopolysaccharide (LPS), laza emva koko lahlalutya umxholo weemveliso ezijikeleze ii-macrophages ngexesha lokuvuvukala. Amanqanaba ee-metabolites ezingama-99 anyusiwe kwaye ii-metabolites ezili-105 zancitshiswa phakathi kwee-molecules ezingama-458 ezifunyenwe ngaphambi nasemva kokuvuselelwa kokuvuvukala, kwaye amanqanaba e-NAD+ ahamba nokuvuvukala nawo ancitshiswa.

(UMFANEKISO 1)

I-NMN yonyusa amanqanaba e-NAD kwaye inciphisa ukudumba kwe-macrophage
Iqela lophando emva koko lanyanga ii-macrophages nge-LPS, nto leyo ebangela imeko yokuvuvukala, i-IL-6 kunye ne-IL-1β, ii-cytokines ezikhuthaza ukuvuvukala ezisebenza njengeempawu zokuvuvukala. Emva konyango lwe-NMN lokuvuvukala kwe-macrophage okubangelwa yi-LPS, kwafunyaniswa ukuba inqanaba le-NAD langaphakathi kwiseli landa kwaye ukubonakaliswa kwe-mRNA kwe-IL-6 kunye ne-IL-1β kwancipha. Uvavanyo lubonise ukuba i-NMN yonyusa inqanaba le-NAD kwaye yanciphisa ukuvuvukala kwe-macrophage okubangelwa yi-LPS.

(Umfanekiso 2)

(Umfanekiso 3)

I-NMN inciphisa amanqanaba eeprotheyini ezinxulumene nokuvuvukala
Unyango lwe-NMN, kwafunyaniswa ukuba iiproteni ezinxulumene nokuvuvukala ezifana ne-RELL1, PTGS2, FGA, FGB kunye ne-igkv12-44 zinciphile kwiiseli, nto leyo ebonisa ukuba i-NMN inciphise ukubonakaliswa kweeproteni ezinxulumene nokuvuvukala.

(Umfanekiso 4)

I-NMN inciphisa ukubonakaliswa kweeproteni ezijoliswe kwi-NSAIDS
Uvavanyo lokugqibela lufumanise ukuba i-NMN yehlise inqanaba le-PGE2 kwiiseli ze-RAW264.7 ezisebenza kwi-LPS ngokunciphisa inqanaba lokubonakaliswa kwe-COX-2, ngaloo ndlela inciphisa ukubonakaliswa kwe-COX2 kwaye ithintele ukudumba okubangelwa yi-LPS.

(Umfanekiso 6)

isiphelo, ukongezwa kwe-NMN kunokunyanga ngempumelelo ukudumba okungapheliyo kwiimpuku, kwaye unyango lokudumba ebantwini kusafuneka luqinisekiswe zizilingo zeklinikhi ezifanelekileyo. Mhlawumbi i-NMN iya kuba yindawo ye-NSAIDS kungekudala.

iireferensi:
I-1.Liu J, i-Zong Z, i-Zhang W, i-Chen Y, i-Wang X, i-Shen J, i-Yang C, i-Liu X, i-Deng H. I-Nicotinamide Mononucleotide Inciphisa i-LPS-Induced Inflammation kunye ne-Oxidative Stress ngokunciphisa i-COX-2 Expression kwi-Macrophages. Ngaphambili Mol Biosci. 2021 Jul 6.