Uphando Express |Uphononongo lweYunivesithi yaseTsinghua lubonisa i-NMN inokunyanga ukudumba

Ukusetyenziswa kwe-Macrophage yindlela ye-pathogenic ekhokelela ekuvukeleni okungapheliyo emzimbeni, kodwa ukusebenza ngokuqhubekayo kwe-macrophage kunokukhokelela ekuvukeleni okungapheliyo kunye nezifo ezifana nokuxhatshazwa kwe-insulin kunye nezifo ezinzima ezifana ne-atherosclerosis.I-PGE 2, edibanisa impendulo yokuvuvukala, yenziwe kwi-arachidonic acid nge-cyclooxygenases (COX-1 kunye ne-COX-2).I-COX-1 kunye ne-COX-2 zezona njongo ziphambili zokuchasana nokudumba kwaye zinokuthintelwa ngamachiza achasene ne-steroidal anti-inflammatory (NSAIDs) .

Ukusetyenziswa kweeNSAID kunokubangela iingxaki ezininzi ezinjengokopha kwesisu .Ngoko ke, kubaluleke kakhulu ukufumana into ekhuselekileyo yendalo ukunyanga ukuvuvukala .

Kutshanje, iqela lophando elivela kwiYunivesithi yaseTsinghua liye laphatha i-macrophages ye-mouse kunye ne-NMN, kwaye yabonisa ngokusebenzisa iimvavanyo ukuba i-NMN inokunciphisa ukuqokelelwa kweeprotheni ezinxulumene nokudumba kunye ne-metabolic byproducts, kwaye inqanda impendulo yokuvuvukala ye-macrophages.Imida kwiBioscience yeMolekyuli .

Ukudumba kuguqula amanqanaba emveliso ye-metabolic kwi-macrophages
Okokuqala, iqela lophando lisebenze i-macrophages ukuvelisa ukuvuvukala ngokusebenzisa i-lipopolysaccharide (LPS) , kwaye emva koko ihlalutye umxholo weeproducts malunga ne-macrophages ngexesha lokuvuvukala.Amanqanaba e-metabolites angama-99 anyuswa kwaye i-metabolites ye-105 yancipha phakathi kwee-molecule ze-458 ezifunyenwe ngaphambi nangemva kokuvuselela ukuvuvukala, kwaye amanqanaba e-NAD + ahamba kunye nokudumba nawo ancipha.

(UMZO 1)

I-NMN inyusa amanqanaba e-NAD kwaye inciphisa ukudumba kwe-macrophage
Iqela lophando laye laphatha i-macrophages kunye ne-LPS, eyenza imeko yokuvuvukala, i-IL-6 kunye ne-IL-1β, i-cytokines e-pro-inflammatory esebenza njengabamakishi bokuvuvukala.Emva kokuba unyango lwe-NMN lwe-LPS-induced macrophage inflammation, kwafunyaniswa ukuba i-intracellular NAD level yanda kwaye i-mRNA ibonakaliso ye-IL-6 kunye ne-IL-1β yancipha.Iimvavanyo zibonise ukuba i-NMN yonyuse inqanaba le-NAD kunye ne-LPS-induced macrophage inflammation.

(Umfanekiso 2)

(Umfanekiso 3)

I-NMN inciphisa amanqanaba eeprotheyini ezinxulumene nokudumba
unyango lwe-NMN, kwafunyaniswa ukuba iiprotheyini ezinxulumene nokuvuvukala ezifana ne-RELL1, i-PTGS2, i-FGA, i-FGB kunye ne-igkv12-44 yancipha kwiiseli, ezibonisa ukuba i-NMN yanciphisa ukubonakaliswa kweeprotheni ezinxulumene nokuvuvukala.

(Umfanekiso 4)

I-NMN inciphisa ukubonakaliswa kweeprotheyini ezijoliswe kwi-NSAIDS
Uvavanyo lokugqibela lufumene ukuba i-NMN yanciphisa izinga le-PGE2 kwiiseli ze-RAW264.7 ezisebenzayo ze-LPS ngokunciphisa inqanaba lokubonisa i-COX-2, ngaloo ndlela inciphisa ukubonakaliswa kwe-COX2 kunye nokuvimbela ukuvuvukala okubangelwa yi-LPS.

(Umfanekiso 6)

isiphetho, ukuxhaswa kwe-NMN kunokunyanga ngokufanelekileyo ukuvuvukala okungapheliyo kwiigundane, kwaye unyango lokuvuvukala ebantwini kusafuneka luqinisekiswe ngamalingo afanelekileyo onyango.Mhlawumbi i-NMN iya kuba endaweni ye-NSAIDS kungekudala.

iimbekiselo:
I-1.Liu J, i-Zong Z, i-Zhang W, i-Chen Y, i-Wang X, i-Shen J, i-Yang C, i-Liu X, i-Deng H. I-Nicotinamide Mononucleotide Inciphisa i-LPS-Induced Inflammation kunye ne-Oxidative Stress ngokunciphisa i-COX-2 Expression kwi-Macrophages.Ngaphambili Mol Biosci.2021 Jul 6.