I-oocyte sisiqalo sobomi bomntu, yiseli yeqanda engekavuthwa ethi ekugqibeleni ikhule ibe liqanda.Nangona kunjalo, umgangatho we-oocyte uyancipha njengoko abasetyhini bekhula okanye ngenxa yezinto ezinjengokutyeba kakhulu, kwaye i-oocyte ekumgangatho ophantsi ngoyena nobangela wokuzala okuphantsi kwabasetyhini abatyebe kakhulu.Nangona kunjalo, indlela yokuphucula umgangatho wee-oocyte kubasetyhini abatyebileyo ngumngeni kwizazinzulu.
Kutshanje, uphononongo olunesihloko esithi Ulawulo lwe-nicotinamide mononucleotide luphucula umgangatho we-oocyte weempuku ezityebileyo lupapashwe kwi-Cell proliferation.Uphononongo lufumanise ukuba ukongezwa kwe-nicotinamide adenine dinucleotide (NAD +) isandulela.nicotinamide mononucleosideI-Acid (NMN) inokuphucula ngokufanelekileyo ukuvuvukala kwe-ovarian, ukuphucula umgangatho we-oocyte kunye nokubuyisela ubunzima bomzimba wenzala kwiigundane ezibhinqileyo.
Abaphandi bakhetha iigundane ezineminyaka eyi-3 ubudala kunye ne-11 - iigundane zesilisa zeveki ukuseka imodeli yemouse yokutyeba ngokutya okunamafutha aphezulu kunye nokuqinisekiswa ngokurekhodwa kwesisindo, ukuhlolwa kwegazi lokuzila ukutya kunye novavanyo lokunyamezela i-glucose yomlomo, ukungenelela kokutya. 1 Kwiiveki ezi-2,NMNizongezo zajojowe kangangeentsuku ezili-10 ezilandelelanayo ukuze kubonwe ukubonakaliswa kofuzo olunxulumene nophuhliso lwe-ovarian kunye nemfuza enxulumene nokuvuvukala, ubungakanani bamafutha ezicubu ze-adipose esiswini, inqanaba le-oxygen esebenzayo ye-oocyte, i-spindlechromosome structure, iMitochondrial Function, actin dynamics, kunye nomonakalo weDNA, xa kuthelekiswa neziphumo zobalo ezibonisa:
1. Ukutya okutyebileyo okutyebileyo okubangelwa yimouse kuye kwasekwa ngempumelelo
Ixabiso le-FGB ye-fat-fat diet (HFD) iqela lalihlala liphezulu kunelo lokutya okuqhelekileyo (ND) iqela, ngaphezu koko, iziphumo ze-OGTT zibonise ukuba ukutya okunamafutha aphezulu (i-HFD) iigundane zeqela zazingenakunyamezela i-glucose.
2. I-NMN inokuphucula ukungahambi kakuhle kwe-metabolic kwiigundane ze-HFD
Ukongezwa ngeNMNukuxhaswa kuncitshiswe umthamo wamafutha kwiigundane kwi-fat-fat diet (HFD) iqela, kwaye iziphumo zibonisa ukuba i-NMN inefuthe lokukhusela kwi-metabolism engaqhelekanga kwiigundane kwi-fat-fat diet (HFD) iqela.
3. I-NMN iphucula umgangatho we-ovarian kwiigundane ze-HFD
I-NMN inokuphucula umgangatho we-ovarian kwi-fat-fat diet (HFD) iigundane ngokulawula ukubonakaliswa kwezakhi zofuzo eziphambili ezinxulumene nophuhliso lwe-ovarian follicle (Bmp4, Lhx8) kunye nokuvuvukala.
4. I-NMN inciphisa i-oocyte division defects kunye nomonakalo we-DNA kwiigundane ze-HFD
I-NMN inokunciphisa i-frequency ephezulu ye-spindle defects kunye ne-chromosomal misalignments ebangelwa kukutya okunamafutha aphezulu ( HFD ), ukunciphisa umqondiso we-γH2A.X, kunye nokuphucula ukutya okunamafutha aphezulu ( HFD ) -ukwenza umonakalo we-DNA ngokunciphisa i-Bax expression.
5. I-NMN inokuphucula umgangatho wee-oocytes
I-NMN inokulungisa ukubonakaliswa kwe-antioxidant SOD1 kwi-high-fat diet (HFD) iqela, ukuphucula ukuhanjiswa kwe-mitochondria, kwaye inokuphucula umgangatho wee-oocyte ngokugcina ingqibelelo ye-cytoskeleton.
6. I-NMN inokubuyisela ukuhanjiswa kwe-lipid droplet kwiigundane ze-HFD
ukutya okunamafutha aphezulu (i-HFD) i-oocyte yeqela yayiphezulu kancinane kunokutya okuqhelekileyo (ND) i-oocyte yeqela, kunye nokuxhaswa kwe-NMN kunokunciphisa ubuninzi be-fluorescence ye-lipid droplets.
7. I-NMN ibuyisela ubunzima bomzimba kwinzala yeempuku ze-HFD
ubunzima bokuzalwa kwenzalo ye-fat-fat diet (HFD) iqela laliphantsi kakhulu kunelo lokutya okuqhelekileyo (ND) iqela, kunye nokuxhaswa nge-NMN yokuxhaswa kwabuyisela ubunzima bokuzalwa benzalo yeqela le-HFD.
Kolu phononongo, abaphandi babonise ngeemvavanyo zempuku ukuba i-NMN inamandla okuphucula umgangatho wee-oocyte kwiimpuku zabasetyhini ezityebileyo ezibangelwa kukutya okunamafutha aphezulu, kwaye batyhila ukuba i-NMN inokubuyisela ukusebenza kwe-mitochondrial kunye nokunciphisa ukuqokelela kwe-ROS kwii-oocyte zemouse ezibhityileyo., Umonakalo we-DNA kunye neendlela ezingaphantsi kwe-lipid droplet distribution.Ke ngoko, olu phononongo luya kubonelela ngesicwangciso sonyango esinokubakho sokuphucula iingxaki zokuzala ezibangelwa kukutyeba kwabasetyhini.
iimbekiselo:
1.Wang L, Chen Y, Wei J, et al.Ulawulo lwe-nicotinamide mononucleotide luphucula umgangatho we-oocyte weempuku ezityebileyo.Iseli Prolif.2022;e13303.doi10.1111cpr.13303
Ixesha lokuposa: Sep-28-2022