I-oocyte sisiqalo sobomi bomntu, liqanda elingavuthwanga eliye ekugqibeleni likhule libe liqanda. Nangona kunjalo, umgangatho we-oocyte uyancipha njengoko abafazi bekhula okanye ngenxa yezinto ezifana nokutyeba kakhulu, kwaye ii-oocyte ezikumgangatho ophantsi zezona zibangela ukungabikho kokuzala kubafazi abatyebileyo. Nangona kunjalo, indlela yokuphucula umgangatho wee-oocyte kubafazi abatyebileyo ngumceli mngeni kwizazinzulu.
Kutshanje, uphando olunesihloko esithi Ulawulo lwe-nicotinamide mononucleotide luphucula umgangatho we-oocyte yeempuku ezityebileyo lupapashwe kwi-Cell proliferation. Olu phononongo lufumanise ukuba ukongezwa kwe-nicotinamide adenine dinucleotide (NAD+) precursori-nicotinamide mononucleosideI-Acid (NMN) inokuphucula ngempumelelo ukudumba kwesibeleko, iphucule umgangatho we-oocyte kwaye ibuyisele ubunzima bomzimba womntwana kwiimpuku eziziimazi ezityebileyo.
Abaphandi bakhethe iimpuku eziziimazi ezineeveki ezi-3 ubudala kunye neempuku eziziinkunzi ezineveki ezili-11 ubudala ukuze bamisele imodeli yempuku yokutyeba ngokutya okunamafutha amaninzi kwaye kuqinisekiswe ngokurekhoda ubunzima, uvavanyo lweswekile yegazi lokuzila ukutya, kunye novavanyo lokunyamezela iswekile ngomlomo, ukungenelela kokutya 1 Kangangeeveki ezi-2,I-NMNIzongezo zifakwe kangangeentsuku ezili-10 ezilandelelanayo ukuze kufunyanwe ukubonakaliswa kwezakhi zofuzo ezinxulumene nophuhliso lwe-ovarian kunye nezakhi zofuzo ezinxulumene nokuvuvukala, ubungakanani bamafutha kwizicubu zamafutha esiswini, inqanaba le-oksijini esebenzayo yee-oocytes, isakhiwo se-spindlechromosome, Umsebenzi we-mitochondrial, i-actin dynamics, kunye nomonakalo we-DNA, xa kuthelekiswa neziphumo zezibalo ezibonisa:
1. Imodeli yegundane etyebileyo ebangelwa kukutya okunamafutha amaninzi yasekwa ngempumelelo
Ixabiso le-FGB leqela le-high-fat diet (HFD) lalihlala liphezulu kunelo leqela le-ND eliqhelekileyo, ukongeza, iziphumo ze-OGTT zibonise ukuba iimpuku zeqela le-high-fat diet (HFD) azikwazi ukumelana ne-glucose.
2. I-NMN inokuphucula ukungasebenzi kakuhle kwe-metabolic kwiimpuku ze-HFD
Ukongezwa kunyeI-NMNukongezwa kunciphisa umthamo wamafutha kwiimpuku kwiqela lokutya okunamafutha amaninzi (HFD), kwaye iziphumo zibonisa ukuba i-NMN inefuthe elikhuselayo kwimetabolism engaqhelekanga kwiimpuku kwiqela lokutya okunamafutha amaninzi (HFD).
3. I-NMN iphucula umgangatho we-ovarian kwiimpuku ze-HFD
I-NMN inokuphucula umgangatho we-ovarian kwiimpuku zokutya okunamafutha amaninzi (HFD) ngokulawula ukubonakaliswa kwezakhi zofuzo ezibalulekileyo ezinxulumene nophuhliso lwe-ovarian follicle (Bmp4, Lhx8) kunye nokuvuvukala.
4. I-NMN inciphisa iziphene zokwahlulwa kwe-oocyte kunye nomonakalo we-DNA kwiimpuku ze-HFD
I-NMN inokunciphisa ukwenzeka okuphezulu kweziphene ze-spindle kunye nokungalingani kwe-chromosome okubangelwa kukutya okunamafutha amaninzi (HFD), inciphise i-γH2A.X signaling, kwaye iphucule umonakalo we-DNA obangelwa kukutya okunamafutha amaninzi (HFD) ngokunciphisa ukubonakaliswa kweBax.
5. I-NMN inokuphucula umgangatho wee-oocytes
I-NMN inokulungisa ukubonakaliswa okuphantsi kwe-antioxidant SOD1 kwiqela le-high-fat diet (HFD), iphucule ukusasazwa kwe-mitochondria, kwaye inokuphucula umgangatho wee-oocytes ngokugcina ukuthembeka kwe-cytoskeleton.
6. I-NMN inokubuyisela ukusasazwa kwe-lipid droplet kwiimpuku ze-HFD
Ii-oocytes zeqela le-diet ezinamafutha aphezulu (HFD) zaziphezulu kancinci kunezo zikwi-oocytes zeqela le-ND yokutya okuqhelekileyo, kwaye ukongezwa kwe-NMN kunokunciphisa amandla e-fluorescence yamaconsi e-lipid.
7. I-NMN ibuyisela ubunzima bomzimba kwinzala yeempuku ze-HFD
Ubunzima bokuzalwa kwenzala yeqela le-HFD yokutya okunamafutha amaninzi babuphantsi kakhulu kunobo beqela le-ND, kwaye ukongezwa nge-NMN ukongezwa kubuyisela ubunzima bokuzalwa kwenzala yeqela le-HFD.
Kolu phononongo, abaphandi babonise ngokusebenzisa uvavanyo lweempuku ukuba i-NMN inamandla okuphucula umgangatho wee-oocytes kwiimpuku eziziinkonde ezityebileyo ezibangelwa kukutya okunamafutha amaninzi, kwaye batyhila ukuba i-NMN inokubuyisela umsebenzi we-mitochondrial kwaye inciphise ukuqokelelwa kwe-ROS kwi-oocytes zempuku eziziinkonde ezityebileyo. , umonakalo we-DNA kunye neendlela ezisisiseko zokusasazwa kwe-lipid droplet. Ke ngoko, olu phononongo luza kubonelela ngesicwangciso esinokwenzeka sonyango sokuphucula iingxaki zokuzala ezibangelwa kukutyeba kakhulu kubasetyhini.
iireferensi:
1.Wang L, Chen Y, Wei J, et al. Ulawulo lwe-nicotinamide mononucleotide luphucula umgangatho we-oocyte yeempuku ezityebileyo. Cell Prolif. 2022; e13303. doi10. 1111cpr.13303
Ixesha lokuthumela: Sep-28-2022