SyncoZymes

iimveliso

I-Carboxylic acid reductase (CAR)

Inkcazelo emfutshane:

Malunga neCarboxylic acid reductase

I-ES-CAR (i-Carboxylic acid reductase): ibangela ukunciphisa iqela le-carboxyl kwiqela le-aldehyde.Kwinkqubo ye-catalytic, ukusebenza kwe-ATP kunye ne-coenzyme NADPH iyafuneka njengabathuthi be-hydrogen.Kukho iintlobo ezi-2 zeemveliso ze-enzyme ze-CAR (Inombolo njenge-ES-CAR101 ~ ES-CAR-102) ephuhliswe ngu-SyncoZymes.

Uhlobo lwempendulo yeCatalytic:

I-Carboxylic acid reductase (CAR)1

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyile:lchen@syncozymes.com


Iinkcukacha zeMveliso

Iithegi zeMveliso

Ulwazi ngeMveliso:

I-Carboxylic acid reductase (CAR)1
Iienzymes Ikhowudi yeMveliso Inkcazo
Ikhithi yokuHlola (SynKit) ES-CAR-101-ES-CAR-102 isethi ye-2 Ketoreductases, i-1 mg nganye yezinto ezi-2 * 1mg / into

Izinto eziluncedo:

★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.

Imiyalelo yokusetyenziswa:

➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer, i-enzyme, i-coenzyme, i-ATP.
➢ ICAR kufuneka yongezwe okokugqibela kwisixokelelwano sokusabela, emva kokuba i-pH kunye nobushushu bulungelelaniswe kwimeko yokusabela.

Imizekelo yosetyenziso:

Umzekelo 1(Ukwenziwa kwecinnamyl alcohol from phenylalanine)(1):

I-Carboxylic acid reductase (CAR)2

Ugcino:

Gcina iminyaka emi-2 ngaphantsi -20 ℃.

Ingqalelo:

Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.

IiReferensi:

1. Zhang, Chen, et al.Iifektri zeeseli zeMicrobial 19.1 (2020): 1-10.


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