IAmine Dehydrogenase (AmDH)
Inkcazelo emfutshane:
Malunga neAmine Dehydrogenase
I-ES-AmDH (i-Amine Dehydrogenase): i-enzyme enokuthi ibangele i-ammoniation yeqela le-carbonyl kwaye iyinciphise kwiqela le-amino.Yi-enzyme exhomekeke kwi-coenzyme ye-NADH, kwaye i-NADH isebenza njenge-hydrogen transmitter.Kukho iintlobo ezi-4 zeemveliso ze-enzyme ye-AmDH (Inombolo njenge-ES-AmDH101～ES-AmDH104) ephuhliswe yi-SyncoZymes, enokusetyenziswa kwi-regioselective okanye i-stereoelective reductive amination of aldehydes and ketones.
Uhlobo lwe-Catalytic reaction:
Iselula/Wechat/WhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.
➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer, i-enzyme, i-coenzyme kunye nenkqubo yokuvuselela i-coenzyme (umz. i-glucose kunye ne-glucose dehydrogenase).
➢ I-AmDH kufuneka yongezwe okokugqibela kwisistim yokusabela, emva kokuba i-pH kunye nobushushu bulungelelaniswe kwimeko yokusabela.
Umzekelo 1(Ulungelelwaniso lweechiral amines ezihambelanayo ngokuncitshiswa kwe-alkyl aryl ketones)(1):
Gcina iminyaka emi-2 ngaphantsi -20 ℃.
Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.
1. Kong W, Liu Y, Huang C, et al.I-Angewandte Chemie International Edition, 2022: e202202264.
