I-D-Amino acid dehydrogenase (D-AADH)
Inkcazo emfutshane:
Malunga ne-D-Amino acid dehydrogenase
I-ES-D-AADH (i-D-Amino acid dehydrogenase): i-enzyme enokunciphisa i-2-keto acid ibe yi-D-amino acid ehambelanayo. I-reaction ifuna i-NADP(H) kunye ne-inorganic ammonia donor (efana ne-ammonia okanye i-ammonium ion). Kukho iintlobo ezili-17 ze-D-amino acid dehydrogenases (Inombolo njenge-ES-DAADH-101~ ES-DAADH-117) eyenziwe yiSyncoZymes.
Uhlobo lwempendulo ye-Catalytic:
Iselfowuni/iWechat/iWhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | ES-D-AADH-101~ ES-D-AADH-117 | iseti ye-17 D-amino Acid Dehydrogenases, 50 mg nganye, izinto ezili-17 * 50mg / into, okanye olunye ubungakanani |
| Isixhobo sokuvavanya (iSynKit) | ES-D-AADH-1700 | iseti yee-D-amino Acid Dehydrogenases ezili-17, 1 mg nganye Izinto ezili-17 * 1mg / into |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Uguquko oluphezulu.
★ Iimveliso ezingaphantsi kwezinye.
★ Iimeko zokusabela okuncinci.
★ Inobuhlobo nokusingqongileyo.
➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka iquke i-substrate, isisombululo se-buffer, i-enzyme, i-coenzyme kunye nenkqubo yokuvuselela i-coenzyme (umz. i-glucose kunye ne-glucose dehydrogenase).
➢ I-D-AADH kufuneka yongezwe okokugqibela kwinkqubo yokusabela, emva kokuba i-pH kunye nobushushu zilungisiwe kwimeko yokusabela.
Umzekelo 1 (Ukunciphisa i-α Ketoacids ukuya kwi-D-amino acids ehambelanayo)(1):
Gcina iminyaka emibini ngaphantsi kwe -20℃.
Ungaze udibane neemeko ezinzima ezifana nobushushu obuphezulu, i-pH ephezulu/ephantsi kunye nesinyibilikisi sendalo esinoxinzelelo oluphezulu.
1. UAkita H, uHayashi J, uSakuraba H, nabanye. I-Frontiers in Microbiology, 2018, 9: 1760.
