I-D-Amino acid dehydrogenase (D-AADH)
Inkcazelo emfutshane:
Malunga ne-D-Amino acid dehydrogenase
I-ES-D-AADH (i-D-Amino acid dehydrogenase): i-enzyme enokunciphisa i-2-keto acid kwi-D-amino acid ehambelanayo.Ukusabela kufuna i-NADP (H) kunye nomxhasi we-ammonia we-inorganic (njenge-ammonia okanye i-ammonium ion).Kukho iintlobo ze-17 ze-D-amino acid dehydrogenases (Inombolo njenge-ES-DAADH-101~ ES-DAADH-117) ephuhliswe yi-SyncoZymes.
Uhlobo lwe-Catalytic reaction:
Iselula/Wechat/WhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | ES-D-AADH-101~ ES-D-AADH-117 | iseti ye-17 D-amino Acid Dehydrogenases, 50 mg nganye 17 izinto * 50mg / into, okanye enye ubungakanani |
| Ikhithi yokuHlola (SynKit) | ES-D-AADH-1700 | iseti ye-17 D-amino Acid Dehydrogenases, 1 mg nganye 17 izinto * 1mg / into |
★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.
➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer, i-enzyme, i-coenzyme kunye nenkqubo yokuvuselela i-coenzyme (umz. i-glucose kunye ne-glucose dehydrogenase).
➢ I-D-AADH kufuneka yongezwe okokugqibela kwisistim yokusabela, emva kokuba i-pH kunye nobushushu bulungelelaniswe kwimeko yokusabela.
Umzekelo 1 (Ukunciphisa i-α Ketoacids ukuya kwi-D-amino acids ehambelanayo)(1):
Gcina iminyaka emi-2 ngaphantsi -20 ℃.
Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.
1. Akita H, Hayashi J, Sakuraba H, et al.Imida kwiMicrobiology, 2018, 9: 1760.
