I-CALB engashukumiyo

Inkcazelo emfutshane:

I-CALB

I-Recombinant Lipase B esuka kwi-Candida antarctica (CALB) iveliswa ngokubiliswa ngaphantsi kwamanzi kunye ne-Pichia pastoris eguqulwe ngofuzo.

I-CALB ingasetyenziselwa kwisigaba samanzi okanye i-organic phase catalytic esterification, i-esterolysis, i-transesterification, i-ring ring polyester synthesis, i-aminolysis, i-hydrolysis ye-amides, i-acylation ye-amines kunye nokuphendula okongeziweyo.

I-CALB inokukhetha okuphezulu kwe-chiral kunye nokukhetha isikhundla, ngoko ingasetyenziselwa ngokubanzi kwi-oyile, ukutya, amayeza, izimonyo kunye namanye amashishini amachiza.

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyile:lchen@syncozymes.com

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

I-CALB engashukumiyo:

I-CALB ayishukunyiswa yi-adsorption ebonakalayo kwi-resin ephezulu ye-hydrophobic eyi-macroporous, i-styrene / i-methacrylate polymer.I-CALB engenakunyakaziswa ifanelekile kwizicelo kwiinkqubo ze-organic solvents kunye ne-solvent-free systems, kwaye ingahlaziywa kwaye isetyenziswe kwakhona amaxesha amaninzi kwiimeko ezifanelekileyo.
Ikhowudi yeMveliso: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.

Izinto eziluncedo ze-SZ-CALB-IMMO100:

★Umsebenzi ophezulu, ukukhetha okuphezulu kwe-chiral kunye nokuzinza okuphezulu.
★Ukusebenza ngcono kwizigaba ezingenamanzi.
★Susa ngokulula kwinkqubo yokusabela, phelisa ngokukhawuleza ukusabela, kwaye unqande intsalela yeprotheyini kwimveliso.
★Inokurisayikilishwa iphinde isetyenziswe ukucutha iindleko.

IiParameters zeMveliso ye-SZ-CALB-IMMO100:

Umsebenzi	≥10000PLU/g
Uluhlu lwe-pH lokusabela	5-9
Uluhlu lobushushu lokusabela	10-60℃
Imbonakalo	I-CALB-IMMO100-A: Umthubi okhanyayo ukuya kumdaka oqinileyo I-CALB-IMMO100-B: Okumhlophe ukuya kumdaka okhanyayo oqinileyo
Ubungakanani besuntswana	300-500μm
Ilahleko ekomisweni kwi-105℃	0.5%-3.0%
Resin for immobilization	Macroporous, styrene/methacrylate polymer
Isinyibilikisi esisabelayo	Amanzi, i-solvent ye-organic, njl., okanye ngaphandle kwe-solvent.Ukuphendula kwezinye izinyibilikisi eziphilayo, amanzi angama-3% anokongezwa ukuphucula umphumo wokuphendula
Ubungakanani besuntswana	I-CALB-IMMO100-A: 200-800 μm I-CALB-IMMO100-B: 400-1200 μm

Inkcazo yeyunithi: Iyunithi ye-1 ihambelana nokuhlanganiswa kwe-1μmol ngomzuzu wepropyl laurate ukusuka kwi-lauric acid kunye ne-1-propanol kwi-60 ℃.I-CALB-IMMP100-A engentla kunye ne-CALB-IMMO100-B ihambelana nabathwali abangenakunyakaziswa kunye nobukhulu obuhlukeneyo.

Imiyalelo kunye noLumkelo:

1. Uhlobo lwe-reactor
I-enzyme engashukumiyo iyasebenza kuzo zombini i-kettle batch reactor kunye nebhedi esisigxina eqhubekayo yokusabela.Kufuneka kuqatshelwe ukuphepha ukutyumza ngenxa yamandla angaphandle ngexesha lokutya okanye ukuzaliswa.
2. Ukusabela pH, ubushushu kunye ne-solvent
I-enzyme ye-immobilized kufuneka yongezwe okokugqibela, emva kokuba ezinye izinto zongezwa kwaye zinyibilike, kunye ne-pH ilungiswe.
Ukuba ukusetyenziswa kwe-substrate okanye ukubunjwa kwemveliso kuya kukhokelela kutshintsho lwe-pH ngexesha lokuphendula, i-buffer eyaneleyo kufuneka yongezwe kwinkqubo yokusabela, okanye i-pH kufuneka ibekwe esweni kwaye ilungiswe ngexesha lokuphendula.
Ngaphakathi koluhlu lokunyamezela ubushushu beCALB (ngaphantsi kwama-60 ℃), izinga lokuguqulwa liye landa ngokunyuka kwamaqondo obushushu.Ukusetyenziswa okusebenzayo, ubushushu bokuphendula kufuneka bukhethwe ngokuhambelana nokuzinza kwe-substrate okanye imveliso.
Ngokuqhelekileyo, i-ester hydrolysis reaction ifanelekile kwinkqubo yesigaba samanzi, ngelixa i-ester synthesis reaction ifanelekile kwinkqubo yesigaba se-organic.I-solvent ye-organic ingaba yi-ethanol, i-tetrahydrofuran, i-n-hexane, i-n-heptane kunye ne-toluene, okanye i-solvent efanelekileyo edibeneyo.Ukuphendula kwezinye izinyibilikisi eziphilayo, amanzi angama-3% anokongezwa ukuphucula umphumo wokuphendula.
3. Ukusetyenziswa kwakhona kunye nobomi benkonzo ye-CALB
Ngaphantsi kwemeko efanelekileyo yokusabela, i-CALB inokufunyanwa kwakhona kwaye isetyenziswe kwakhona, kwaye amaxesha athile okufaka isicelo ayahluka kwiiprojekthi ezahlukeneyo.
Ukuba i-CALB efunyenweyo ayiphinda isetyenziswe ngokuqhubekayo kwaye kufuneka igcinwe emva kokuchacha, kufuneka ihlanjwe yomiswe kwaye ivalwe kwi-2-8 ℃.
Emva kwemijikelo emininzi yokusetyenziswa kwakhona, ukuba ukusebenza kakuhle kokusabela kuncitshiswe kancinane, i-CALB inokongezwa ngokufanelekileyo kwaye iqhubeke isetyenziswa.Ukuba ukusebenza kakuhle kokusabela kuncitshiswe ngokunzulu, kufuneka kutshintshwe.

Imizekelo yosetyenziso:

Umzekelo 1(Aminolysis)⁽¹⁾:

Umzekelo 2(Aminolysis)⁽²⁾:

Umzekelo 3(Iringi yokuvula ipolyester synthesis)⁽³⁾:

Umzekelo 4(Transesterification, regioselective of hydroxyl group)⁽⁴⁾:

Umzekelo 5(Ukuguqulwa, isisombululo sekinetic yotywala be-racemic)⁽⁵⁾:

Umzekelo 6(I-Esterification, isisombululo sekinetic se-carboxylic acid)⁽⁶⁾:

Umzekelo 7(I-Esterolysis, isisombululo sekinetic)⁽⁷⁾:

Umzekelo 8(Hydrolysis of amides)⁽⁸⁾:

Umzekelo 9(Acylation of amines)⁽⁹⁾:

Umzekelo 10(Aza-Michael addition reaction)⁽¹⁰⁾:

IiReferensi:

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