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SyncoZymes

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Ene reductase (ERED) Umfanekiso okhoyo
  • I-Ene reductase (ERED)

I-Ene reductase (ERED)

Inkcazelo emfutshane:

Malunga ne-ene reductase

I-ES-EREDs: Ivela kwintsapho ye-enzymes endala ephuzi ekhetha ukunciphisa i-C = C kwi-α, i-β -i-unsaturated compounds kunye namaqela athatha i-electron.Zisetyenziswa ngokubanzi kuphononongo lwe-amino acid derivatives, izinto eziphilayo, i-pharmaceutical intermediates, iziqholo kunye neebhloko ze-chiral.

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyile:lchen@syncozymes.com


Iinkcukacha zeMveliso

Iithegi zeMveliso

Malunga ne-ene reductase:

I-ES-EREDs zenza iintlobo ngeentlobo zesubstrate ngenxa yobuninzi besubstrates.Ngokuqhelekileyo, i-C = C ye-α, i-β -unsaturated compounds kunye namaqela e-electron-absorbing (kuquka i-ketone aldehyde, amaqela e-nitro, i-carboxylic acids, i-esters, i-anhydride, i-lactones, imines, njl.) iyancipha ngokulula yi-ES-EREDs, kodwa iibhondi eziphindwe kabini ezingasetyenziswanga.

Kukho iintlobo ze-46 zeemveliso ze-enzyme ze-ERED (Inombolo njenge-ES-ERED-101~ES-ERED-146) ephuhliswe yi-SyncoZymes.

Indlela yokwenza:

Ene ukunciphisa ERED1

Ulwazi ngeMveliso:

Ene reductase ERED2
Iienzymes Ikhowudi yeMveliso Inkcazo
Umgubo we-Enzyme ES-ERED-101~ ES-ERED-146 iseti ye-46 Ene Reductases, 50 mg nganye 46 izinto * 50mg / into, okanye enye ubungakanani
Ikhithi yokuHlola (SynKit) ES-ERED-4600 iseti ye-46 Ene Reductases, 50 mg nganye 46 izinto * 50mg / into, okanye enye ubungakanani

Izinto eziluncedo:

★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.
★ ukhuseleko oluphezulu.

Imiyalelo yokusetyenziswa:

➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer (optimum reaction pH), i-coenzymes (NAD (H) okanye i-NADP (H) ), inkqubo yokuvuselela i-coenzyme (umzekelo, i-glucose kunye ne-glucose dehydrogenase) kunye ne-ES-ERED.
➢ Zonke ii-ES-EREDs zinokuvavanywa ngokulandelelanayo kwi-reaction system engentla okanye nge-ERED Screening Kit (SynKit ERED).
➢ Zonke iintlobo ze-ES-ERED ezihambelana neemeko ezahlukeneyo zokusabela kufuneka zifundwe zodwa.
➢ Uxinzelelo oluphezulu lweSubstrate okanye imveliso enento enokuthintela umsebenzi weES-ERED.Nangona kunjalo, isithintelo sinokukhululeka ngokongezwa kwebhetshi ye-substrate.

Imizekelo yosetyenziso:

Umzekelo 1(α,β-unsaturated aldehydes okanye iiketones)(1):

Ene reductase ERED3

Umzekelo 2(α,β-unsaturated carboxylic acids kunye nezinto eziphuma kuzo)(2):

Ene reductase ERED4

Ugcino:

Gcina iminyaka emi-2 ngaphantsi -20 ℃.

Ingqalelo:

Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.

IiReferensi:

1. Lucídio C, Fardelone J, Augusto R, e tal.J.Mol.Catal.B:Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, e tal..Org.Lett, 2007, 9(26): 5409-5411.

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