I-Cytochrome P450 monooxygenase (CYP)

Inkcazelo emfutshane:

Malunga neCYP

I-ES-CYPs: Iklasi ye-redox enzymes kunye ne-mercaptan-heme njengeziko elisebenzayo lokunyusa ukuhanjiswa kwe-oxygen.Ziyi-oxygen e-terminal kwaye idinga i-heme ukuze isebenze i-oxygen.

I-Cytochrome P450 monooxygenase yenza i-oxidation, i-epoxidation kunye ne-hydroxylation ye-alkyl, i-hydroxylation kunye ne-oxidation ye-ammonia, i-oxidation yesulfure, i-dealkylation (i-oxygen, i-sulfur, i-ammonia), i-oxidative dehydrogenation, i-deamination kunye ne-dehalogenation.
Kukho iintlobo ezisi-8 zeemveliso ze-cytochrome P450 monooxygenase (Inombolo njenge-ES-CYP-101～ES-CYP-108) ephuhliswe ngu-SyncoZymes.

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyile:lchen@syncozymes.com

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Malunga neCYP:

Uhlobo lwe-Catalytic reaction:

Ulwazi ngeMveliso:

Iienzymes	Ikhithi yokuHlola (SynKit)	Inkcazo
Umgubo we-Enzyme	ES-CYP-101~ ES-CYP-108	iseti ye-8 Cytochrome P450 Monooxygenases, 50 mg nganye 8 izinto * 50mg / into, okanye enye ubungakanani
Ikhithi yokuHlola (SynKit)	ES-CYP-800	iseti ye-8 Cytochrome P450 Monooxygenases, 1mg nganye 8 izinto * 1mg / into

Izinto eziluncedo:

★ I-spectrum ebanzi yesubstrate.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.

Imiyalelo yokusetyenziswa:

➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer (I-optimum reaction pH ye-enzyme), i-coenzyme (NAD (H) okanye i-NADP (H)), inkqubo yokuvuselela i-coenzyme (umzekelo, i-glucose kunye ne-glucose dehydrogenase) kunye ne-ES-CYP.I-coenzyme kunye nenkqubo yokuvuselela i-coenzyme inokutshintshwa yi-hydrogen peroxide kwinkqubo yokusabela ngokungaphelelanga.
➢ Zonke iintlobo zee-ES-CYPs ezihambelana neemeko ezahlukeneyo zokusabela kufuneka zifundwe zodwa.
➢ Uxinzelelo oluphezulu lweSubstrate okanye imveliso enento enokuthi ithintele umsebenzi weES-CYP.Nangona kunjalo, isithintelo sinokukhululeka ngokongezwa kwebhetshi ye-substrate.

Imizekelo yosetyenziso:

Umzekelo 1⁽¹⁾:

Umzekelo 2⁽²⁾:

Umzekelo 3⁽³⁾:

Umzekelo 4⁽⁴⁾:

Ugcino:

Gcina iminyaka emi-2 ngaphantsi -20 ℃.

Ingqalelo:

Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.

IiReferensi:

1. Zaretzki J, Matlock M, kunye ne-Swamidass S JJ Chem.Inf.Umzekelo, 2013, 53, 3373-3383.
2. Gannett P M., Kabulski J, Perez F A., e tal.J. Am.Ikhem.Soc., 2006, 128 (26), 8374-8375.
3. UCryle M J., uMatovic N J., kunye noDe Voss J J. Org.Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal.Biochim.I-Biophys.Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.;Cheon, WK e tal.Acta Crystallog.2009, 65, 372.