IHydroaminase (HAM)
Inkcazelo emfutshane:
Malunga neHydroaminase
Kukho iintlobo ezi-2 zemveliso ye-enzyme ye-HAM (Inani njenge-ES-HAM-101~ ES-HAM-102) ephuhliswe yi-SyncoZymes.I-HAM inokwenza i-ammoniation ye-enoic acid okanye i-derivatives yayo ivelise i-chiral amino acids.I-HAM ingasetyenziselwa ukwenza i-chiral amino acids (okanye i-chiral amines) ukusuka kwi-enoic acids (okanye i-alkenes).Umnikezeli we-ammonia, njengamanzi ammonia okanye ityuwa ye-ammonium, iyimfuneko.
IHydroaminase uhlobo lokusabela olusebenzayo
Iselula/Wechat/WhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | ES-HAM-101~ ES-HAM-102 | iseti ye-2 Nuclosidephosphoryalse, 50 mg nganye 2 izinto * 50mg / into, okanye enye ubungakanani |
Le ndlela yokusabela ilandelayo ingasetyenziselwa ireferensi:
I-1-10 mg / ml: I-Substrate
10 mg/ml: I-Enzyme
I-10% (v/v): i-Organic cosolvent (1)
I-10% (v/v): I-Amoniya
Lungisa i-pH kwi-9.0 ngaphambi kokuba ungeze i-enzyme, kwaye i-enzyme kufuneka yongezwe ekugqibeleni.Fuka iintshukumo kwi-shaker yobushushu engaguqukiyo (umzekelo, 30oC, 150rpm) ngeeyure ezingama-24~48.I-Centrifuge umxube ngamnye ukuya kwiprotheni ephantsi, i-supernatant ingasetyenziselwa i-TLC, i-HPLC okanye uhlalutyo lwe-GC.
Qaphela (1): I-solvent ye-Organic (efana ne-DMSO, i-methanol, i-acetonitrile, i-IPA, njl.) iyakhethwa, isetyenziselwa i-substrates e-insoluble.
