I-Hydroaminase (i-HAM)
Inkcazo emfutshane:
Malunga neHydroaminase
Kukho iintlobo ezimbini zemveliso ye-enzyme ye-HAM (Inombolo njenge-ES-HAM-101~ ES-HAM-102) eyenziwe yiSyncoZymes. I-HAM inokwenza i-ammonia ye-enoic acid okanye izinto eziphuma kuyo zivelise i-chiral amino acids. I-HAM ingasetyenziselwa ukwenza i-chiral amino acids (okanye i-chiral amines) kwi-enoic acids (okanye i-alkenes). I-Ammonia donor, efana namanzi e-ammonia okanye ityuwa ye-ammonium, iyimfuneko.
Uhlobo lwempendulo ye-Hydroaminase catalytic
Iselfowuni/iWechat/iWhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | ES-HAM-101~ ES-HAM-102 | iseti yeeNuclosidephosphorylse ezi-2, 50 mg nganye 2 izinto * 50mg / into, okanye olunye ubungakanani |
Le nkqubo ilandelayo yokusabela ingasetyenziselwa ukubhekisa:
1-10 mg/ml: I-Substrate
10 mg/ml: I-Enzyme
10% (v/v): I-organic cosolvent (1)
10% (v/v): I-Ammonia
Lungisa i-pH ibe yi-9.0 ngaphambi kokongeza i-enzyme, kwaye i-enzyme kufuneka yongezwe ekugqibeleni. Faka ii-reactions kwi-temperature shaker engaguqukiyo (umzekelo, i-30oC, i-150rpm) iiyure ezingama-24 ukuya kuma-48. Faka i-centrifuge kumxube ngamnye ukuze ulungelelanise iproteni, apho i-supernatant ingasetyenziselwa uhlalutyo lwe-TLC, HPLC okanye i-GC.
Qaphela (1): Isinyibilikisi sendalo (esifana ne-DMSO, i-methanol, i-acetonitrile, i-IPA, njl.njl.) asinyanzelekanga, sisetyenziselwa izinto ezinganyibilikiyo.
