I-Nitrile reductase (NRED)

Inkcazelo emfutshane:

Malunga neNitrile reductase

Kukho uhlobo lwe-3 yemveliso ye-enzyme ye-NRED (Inombolo njenge-ES-NRED101~ ES-NRED103) ephuhliswe ngu-SyncoZymes.I-NRED inokubangela ukuncipha kweqela le-nitrile kwi-amine.Kwinkqubo ye-catalysis, i-NADPH iyafuneka njenge-hydrogen transporter.

Uhlobo lokusabela lwe-Nitrile reductasecatalytic

I-Nitrile reductase NRED2

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyile:lchen@syncozymes.com

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ulwazi ngeMveliso:

Iienzymes	Ikhowudi yeMveliso	Inkcazo
Inkcazo	ES- NRED -101~ ES- NRED -103	iseti ye-3 Nitrile Reductase, 50 mg nganye 3 izinto * 50mg / into, okanye enye ubungakanani

Inkqubo yokuhlolwa kwe-nitrile reductase yokusabela:

Le ndlela yokusabela ilandelayo ingasetyenziselwa ireferensi:
I-1-10 mg / ml Substrate
10 mg / ml Enzyme
I-10% (v/v) i-Organic cosolvent (1)
100 mM isithinteli sePhosphate (pH7.0)
0.2 mg / ml NADP +
I-10 mg / ml iGlucose
2 mg/ml iGlucose dehydrogenase (GDH)
Fuka iintshukumo kwi-shaker yobushushu engaguqukiyo (umzekelo, 30oC, 150rpm) ngeeyure ezingama-24~48.I-Centrifuge umxube ngamnye ukuya kwiprotheni ephantsi, i-supernatant ingasetyenziselwa i-TLC, i-HPLC okanye uhlalutyo lwe-GC.
Qaphela (1): I-solvent ye-Organic (efana ne-DMSO, i-methanol, i-acetonitrile, i-IPA, njl.) iyakhethwa, isetyenziselwa i-substrates e-insoluble.