I-Lactate Dehydrogenase (LDH)
Inkcazelo emfutshane:
Malunga neLactate dehydrogenase
Kukho uhlobo lwe-1 lwemveliso ye-enzyme ye-LDH (Inombolo njenge-ES-LDH) ephuhliswe yi-SyncoZymes.I-LDH inokubangela ukunciphisa i-pyruvate kwi-L-lactic acid, okanye ukunciphisa ezinye i-α-keto acids ezifanayo.Kwinkqubo ye-catalysis, i-NADH iyimfuneko njenge-hydrogen transporter.
Lactate dehydrogenasecatalytic reaction type
Iselula/Wechat/WhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | ES-LDH-101 | iseti ye-1 Monoamine Oxidase, 50 mg nganye 1 izinto * 50mg / into, okanye enye ubungakanani |
Le ndlela yokusabela ilandelayo ingasetyenziselwa ireferensi:
I-1-10 mg / ml: I-Substrate
10 mg/ml: I-Enzyme
I-10% (v/v): i-Organic cosolvent (1)
100 mM: Isithinteli sePhosphate (pH7.0)
0.2 mg / ml: NAD +
100 mM: Ifomati yeammonium
10 mg/ml: Formate dehydrogenase (FDH)
Fuka iintshukumo kwi-shaker yobushushu engaguqukiyo (umzekelo, 30oC, 150rpm) ngeeyure ezingama-24~48.I-Centrifuge umxube ngamnye ukunyusa iprotheni, i-supernatant ingasetyenziselwa i-TLC, i-HPLC okanye uhlalutyo lwe-GC.
Qaphela (1): I-solvent ye-Organic (efana ne-DMSO, i-methanol, i-acetonitrile, i-IPA, njl.) iyakhethwa, isetyenziselwa i-substrates e-insoluble.
