ILeucine Dehydrogenase (LeuDH)
Inkcazelo emfutshane:
Malunga neLeucine dehydrogenase
Kukho uhlobo lwe-1 lwemveliso ye-enzyme ye-LeuDH (Inombolo njenge-ES-LeuDH-101) ephuhliswe ngu-SyncoZymes.I-LeuDH inokwenza i-ammoniation yokunciphisa i-4-methylbutanoic acid (ityuwa) kwi-L-leucine okanye i-oxidation ye-leucine kwi-4-methylbutanoic acid.I-ES-LeuDH inokwenza kwakhona i-amination yokunciphisa i-α-keto acids enesakhiwo esifanayo ukulungiselela i-amino acid derivatives ehambelanayo.Kwinkqubo ye-catalysis, i-NADH iyimfuneko njenge-hydrogen transporter.
Leucine dehydrogenasecatalytic reaction type
Iselula/Wechat/WhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | ES-LeuDH-101 | iseti ye-1 Monoamine Oxidase, 50 mg nganye 1 izinto * 50mg / into, okanye enye ubungakanani |
Le ndlela yokusabela ilandelayo ingasetyenziselwa ireferensi:
I-1-10 mg / ml: I-Substrate
10 mg/ml: I-Enzyme
I-10% (v/v): i-Organic cosolvent (1)
100 mM: Isithinteli sePhosphate (pH7.0)
0.2 mg / ml: NAD +
100 mM: Ifomati yeammonium
10 mg/ml: Formate dehydrogenase (FDH)
Fuka iintshukumo kwi-shaker yobushushu engaguqukiyo (umzekelo, 30oC, 150rpm) ngeeyure ezingama-24~48.I-Centrifuge umxube ngamnye ukunyusa iprotheni, i-supernatant ingasetyenziselwa i-TLC, i-HPLC okanye uhlalutyo lwe-GC.
Qaphela (1): I-solvent ye-Organic (efana ne-DMSO, i-methanol, i-acetonitrile, i-IPA, njl.) iyakhethwa, isetyenziselwa i-substrates e-insoluble.
