Phosphokinase (PKase)

Inkcazelo emfutshane:

Malunga nePhosphokinase

I-ES-PKase (i-Phosphokinase): ibangela ukuhanjiswa kwamaqela e-phosphate kwi-ATP kwezinye iikhompawundi.Ikwadala ukudluliselwa kwamaqela ephosphate kwezinye iinucleosides zetriphosphate ngamanye amaxesha.Uninzi lwe-kinases ludinga i-ion zetsimbi ze-divalent ukuba zithathe inxaxheba ekuphenduleni (ngokubanzi i-Mg2 +).Kukho iintlobo ze-21 zeemveliso ze-enzyme ze-PCase (Inombolo njenge-ES-PKase101~ ES-PKase-121) ephuhliswe yi-SyncoZymes.
Uhlobo lwe-Catalytic reaction:

Phosphokinase PKase2

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyile:lchen@syncozymes.com

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ulwazi ngeMveliso:

Iienzymes	Ikhowudi yeMveliso	Inkcazo
Ikhithi yokuHlola (SynKit)	ES-PKase-101~ES-PKase-121	isethi ye-21 Ketoreductases, i-1 mg nganye yezinto ze-21 * 1mg / into

Izinto eziluncedo:

★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.

Imiyalelo yokusetyenziswa:

➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer, i-enzyme, i-ATP, i-Mg²⁺.
➢ I-PCase kufuneka yongezwe okokugqibela kwisistim yokusabela, emva kokuba i-pH kunye nobushushu bulungelelaniswe kwimeko yokusabela.

Imizekelo yosetyenziso:

Umzekelo 1(I-Synthesis ye-nicotinamide ribose phosphate esuka kwi-nicotinamide ribose)⁽¹⁾:

Qaphela: imizekelo yesicelo kunye nezikhombisi zenzelwe ukubonisa umda wesicelo se-PCase ukuze kube lula ukuqonda, kwaye ayihambelani ne-enzyme ethile ye-SyncoZymes.