I-Leucine Dehydrogenase (LeuDH)
Inkcazo emfutshane:
Malunga neLeucine dehydrogenase
Kukho uhlobo olunye lwemveliso ye-enzyme yeLeuDH (Inombolo njenge-ES-LeuDH-101) eyenziwe yiSyncoZymes. I-LeuDH inokwenza i-ammoniation ye-4-methylbutanoic acid (ityuwa) ibe yi-L-leucine okanye i-oxidation ye-leucine ibe yi-4-methylbutanoic acid. I-ES-LeuDH ingenza i-α-keto acids ibe yi-reductive amination enesakhiwo esifanayo ukulungiselela i-amino acid derivatives ehambelanayo. Kwinkqubo ye-catalysis, i-NADH iyimfuneko njengomthuthi we-hydrogen.
Uhlobo lwempendulo ye-Leucine dehydrogenasecatalytic
Iselfowuni/iWechat/iWhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | I-ES-LeuDH-101 | iseti ye-1 Monoamine Oxidase, 50 mg nganye 1 into * 50mg / into, okanye olunye ubungakanani |
Le nkqubo ilandelayo yokusabela ingasetyenziselwa ukubhekisa:
1-10 mg/ml: I-Substrate
10 mg/ml: I-Enzyme
10% (v/v): I-organic cosolvent (1)
100 mM: I-Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: I-Ammonium formate
10 mg/ml: I-Formate dehydrogenase (FDH)
Faka ii-reactions kwi-temperature shaker engaguqukiyo (umzekelo, i-30oC, i-150rpm) iiyure ezingama-24 ukuya kuma-48. Faka i-centrifuge kumxube ngamnye ukuze ulungelelanise iproteni, apho i-supernatant ingasetyenziselwa uhlalutyo lwe-TLC, i-HPLC okanye i-GC.
Qaphela (1): Isinyibilikisi sendalo (esifana ne-DMSO, i-methanol, i-acetonitrile, i-IPA, njl.njl.) asinyanzelekanga, sisetyenziselwa izinto ezinganyibilikiyo.
