SyncoZymes

iimveliso

Formate dehydrogenase (FDH)

Inkcazelo emfutshane:

Malunga neFomate dehydrogenase

I-ES-FDH (i-Formate dehydrogenase): I-FDH yenza i-dehydrogenation ye-ammonium formate ukuvelisa i-carbon dioxide kunye ne-ammonia, kunye nokunciphisa i-NAD kwi-NADH kunye ne-NAD njenge-electron receptor.I-FDH isoloko isetyenziselwa ukuhlaziywa kwe-coenzyme NADH.Kukho iintlobo ezi-4 zeemveliso ze-enzyme ze-FDH (ES-FDH-101~ES-FDH-104) eziphuhliswe yi-SyncoZymes, phakathi kwazo i-ES-FDH102 inomsebenzi ophezulu kwaye yeyona FDH ecetyiswayo.

Uhlobo lwe-Catalytic reaction:

Formate dehydrogenase (FDH)1

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I-imeyile:lchen@syncozymes.com


Iinkcukacha zeMveliso

Iithegi zeMveliso

Ulwazi ngeMveliso:

Formate dehydrogenase (FDH)0
Iienzymes Ikhowudi yeMveliso Inkcazo
Umgubo we-Enzyme ES-FDH-101~ ES-FDH-104 iseti ye-4 Epoxide Hydrolases, 50 mg nganye 4 izinto * 50mg / into, okanye enye ubungakanani

Izinto eziluncedo:

★ Ubucacileyo besubstrate ephezulu.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.

Imiyalelo yokusetyenziswa:

➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer, i-enzyme, kunye ne-coenzyme.
➢ Ukuba i-FDH isetyenziselwa ukuvuselelwa kwe-coenzyme, i-enzyme ephambili iyimfuneko, kwaye inkqubo yokusabela kufuneka yenziwe ngokwe-enzyme ephambili.

Imizekelo yosetyenziso:

Umzekelo 1(Ubungakanani beNAD)(1):

Formate dehydrogenase (FDH)2

Umzekelo 2(Biocatalysis)(2):

Formate dehydrogenase (FDH)3

Ugcino:

Gcina iminyaka emi-2 ngaphantsi -20 ℃.

Ingqalelo:

Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.

IiReferensi:

1. Scheller PN, Fademrecht S, Hofelzer S, et al.I-ChemBioChem, i-2014, i-15, i-2201-2204.
2. Wetzl D, Gand M, Ross A, et al.ChemCatChem, 2016, 8, 2023-2026.
3. U-Li H, uLuan ZJ, uZheng GW, et al.Adv.Synth.I-Catal.2015, 357, 1692-1696.


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