I-Lysine oxidase (LO)
Iienzymes | Ikhowudi yeMveliso | Inkcazo |
Umgubo we-Enzyme | ES-LO-101 | isethi ye-1 Ketoreductases, 50 mg nganye 1 izinto * 50mg / into, okanye enye ubungakanani |
★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.
➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer, i-enzyme.
➢ I-LO kufuneka yongezwe okokugqibela kwisistim yokusabela, emva kokuba i-pH kunye nobushushu bulungelelaniswe kwimeko yokusabela.
Umzekelo 1(I-Synthesis ye-L-2-pipecolic acid esuka kwi-lysine)(1):
Gcina iminyaka emi-2 ngaphantsi -20 ℃.
Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.
1. UTani, uYasushi, et al.I-Microbiology esetyenzisiweyo kunye ne-biotechnology 99.12 (2015): 5045-5054.