SyncoZymes

iimveliso

I-Cyclohexanone monooxygenase (CHMO)

Inkcazelo emfutshane:

Malunga neCyclohexanone monooxygenase

I-ES-CHMO (i-Cyclohexanone monooxygenase): i-Baeyer Villiger monooxygenase (BVMO) kunye ne-cyclohexanone njenge-substrate, i-oxidize i-cyclohexanone kwi-cyclohexanol isebenzisa i-oxygen.

Luhlobo lwe-enzyme exhomekeke kwi-flavin.I-flavin ene-oxidized iyancitshiswa ibe yi-flavin ngomjikelezo wokunciphisa i-coenzyme II (NADPH) kwi-oxidation elandelayo.Ke ngoko, i-cofactor NADPH iyimfuneko kumjikelo weflavin.Kukho kuphela uhlobo lwe-1 lwemveliso ye-enzyme ye-CHMO (Inombolo njenge-ES-CHMO101) ephuhliswe yi-SyncoZymes, engasetyenziselwa i-oxidize i-cyclohexanone kwi-cyclohexanone.

Uhlobo lwempendulo yeCatalytic:

Cyclohexanone monooxygenase CHMO1

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyile:lchen@syncozymes.com


Iinkcukacha zeMveliso

Iithegi zeMveliso

Ulwazi ngeMveliso:

Cyclohexanone monooxygenase CHMO2
Iienzymes Ikhowudi yeMveliso Inkcazo
Ikhithi yokuHlola (SynKit) ES-CHMO-101 isethi ye-1 Ketoreductases, 1 mg nganye 1 izinto * 1mg / into

Izinto eziluncedo:

★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.

Imiyalelo yokusetyenziswa:

➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer, i-enzyme, i-coenzyme kunye nenkqubo yokuvuselela i-coenzyme (umz. i-glucose kunye ne-glucose dehydrogenase).
➢ I-CHMO kufuneka yongezwe okokugqibela kwisistim yokusabela, emva kokuba i-pH kunye nobushushu bulungelelaniswe kwimeko yokusabela.

Imizekelo yosetyenziso:

Umzekelo 1(I-Synthesis ye-cyclohexanol esuka kwi-cyclohexanol)(1):

Cyclohexanone monooxygenase CHMO3

Ugcino:

Gcina iminyaka emi-2 ngaphantsi -20 ℃.

Ingqalelo:

Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.

IiReferensi:

1. Friso, Marco, Appl Microbiol Biotechnol, 2017, 101 (20): 7557-7565


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