I-Nitrile hydratase (NHT)
Inkcazelo emfutshane:
Malunga ne-ES-NHTs
I-ES-NHTs: iklasi ye-metalloenzyme, ekhetha i-hydrate nitriles kwi-amide ephambili ehambelanayo.Asetyenziswa ngokubanzi kungekuphela nje ukuhlanganiswa kwe-amino acids, i-amides, i-carboxylic acid kunye ne-derivatives yazo, kodwa kunye nokulungiselela iziyobisi ze-chiral.Kukho iindidi ezingama-24 ze-nitrile hydratases eziphuhliswe yi-SyncoZymes (Inombolo njenge-ES-NHT-101～ES-NHT-124).
Uhlobo lwe-Catalytic reaction:
Iselula/Wechat/WhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | ES-NHT-101~ ES-NHT-124 | iseti ye-24 ye-Nitrile Hydratases, 50 mg nganye 24 izinto * 50mg / into, okanye enye ubungakanani |
| Ikhithi yokuHlola (SynKit) | ES-NHT-2400 | iseti ye-24 ye-Nnitrile Hydratases, i-1 mg nganye izinto ezingama-24 * 1mg / into |
★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.
➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer (optimum reaction pH) kunye ne-ES-NHT.
➢ Zonke ii-ES-NHTs zinokuvavanywa ngokulandelelanayo kwinkqubo yokusabela engentla okanye nge-NHT Screening Kit (SynKit NHT).
➢ Zonke iintlobo ze-ES-NHTs ezingqamana neemeko ezahlukeneyo zokusabela kufuneka zifundwe zodwa.
➢ Uxinzelelo oluphezulu lweSubstrate okanye imveliso enento enokuthi ithintele ukusebenza kwe-ES-NHT.Nangona kunjalo, ukuvinjelwa kunokukhululwa ngokudibanisa kwe-batch ye-substrate, ukudibanisa ne-amidases okanye ukukhutshwa kwemveliso.
Umzekelo 1(ukudibanisa ne-amidase)(1):
Umzekelo 2(2):
Gcina iminyaka emi-2 ngaphantsi -20 ℃.
Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.
1 Vojtech V, Ludmila M, Alicja BV, e tal.J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, etal.Tetrahedron lett, 2010, 51: 1639-1641.
