I-Nitrile hydratase (NHT)
Inkcazo emfutshane:
Malunga ne-ES-NHTs
Ii-ES-NHTs: udidi lwe-metalloenzyme, olukhetha ukufunxa ii-nitriles kwi-primary amide ehambelanayo. Zisetyenziswa kakhulu kungekuphela nje ekuhlanganiseni ii-amino acids, ii-amides, ii-carboxylic acids kunye ne-derivatives zazo, kodwa nasekulungiseleleni amayeza e-chiral. Kukho iintlobo ezingama-24 ze-nitrile hydratases eziphuhliswe yiSyncoZymes (Inombolo njenge-ES-NHT-101～ES-NHT-124).
Uhlobo lwempendulo ye-Catalytic:
Iselfowuni/iWechat/iWhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | ES-NHT-101~ ES-NHT-124 | iseti yama-24 eNitrile Hydratases, ama-50 mg nganye, izinto ezingama-24 * 50mg / into, okanye olunye ubungakanani |
| Isixhobo sokuvavanya (iSynKit) | I-ES-NHT-2400 | iseti yee-Nnitrile Hydratases ezingama-24, i-1 mg nganye, izinto ezingama-24 * 1mg / into |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Uguquko oluphezulu.
★ Iimveliso ezingaphantsi kwezinye.
★ Iimeko zokusabela okuncinci.
★ Inobuhlobo nokusingqongileyo.
➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka iquke i-substrate, isisombululo se-buffer (i-pH yempendulo efanelekileyo) kunye ne-ES-NHT.
➢ Zonke ii-ES-NHTs zingavavanywa ngokulandelelana kwinkqubo yokusabela engentla okanye nge-NHT Screening Kit (SynKit NHT).
➢ Zonke iintlobo ze-ES-NHTs ezihambelana neemeko ezahlukeneyo zokusabela ezifanelekileyo kufuneka zifundwe nganye nganye.
➢ Uxinzelelo oluphezulu lwe-Substrate okanye imveliso enayo inokuthintela umsebenzi we-ES-NHT. Nangona kunjalo, uthintelo lunokupheliswa ngokongeza i-batch substrate, ngokudibana ne-amidases okanye ukukhupha imveliso.
Umzekelo 1 (ukudibana ne-amidase)(1):
Umzekelo 2(2):
Gcina iminyaka emibini ngaphantsi kwe -20℃.
Ungaze udibane neemeko ezinzima ezifana nobushushu obuphezulu, i-pH ephezulu/ephantsi kunye nesinyibilikisi sendalo esinoxinzelelo oluphezulu.
1 Vojtech V, Ludmila M, Alicja BV, e tal. J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, et tal. I-Tetrahedron lett, 2010, 51: 1639-1641.
