Phenylalanine dehydrogenase (PDH)
Inkcazelo emfutshane:
Malunga nePhenylalanine dehydrogenase
ES-PDH: Iklasi ye-oxidoreductase.Ukusabela okufanelekileyo kunokubangela i-oxidative deamination ye-L phenylalanine ityuwa phambi kwe-NAD ukuvelisa ityuwa ye-phenylpyruvate, kunye ne-reverse reaction can catalyze synthesis ye-amino acid ityuwa ehambelana netyuwa ye-phenylpyruvate.I-Syncozymes iphuhlise izinto ze-8 ze-phenylalanine dehydrogenase (inombolo ye-ES-PDH-101~ES-PDH-108).I-ES-PDH ingasetyenziselwa ukulungiselela ityuwa ye-phenylalanine okanye ityuwa ye-amino acid ehambelanayo esuka kwityuwa ye-phenylpyruvic okanye i-derivatives.
Uhlobo lwe-Catalytic reaction:
Iselula/Wechat/WhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | ES-PDH-101~ ES-PDH-108 | iseti ye-8 Nitrile Reductase, 50 mg nganye 8 izinto * 50mg / into, okanye enye ubungakanani |
★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.
➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer, i-enzyme, i-coenzyme (i-NAD (H)), inkqubo yokuvuselela i-coenzyme (umz. i-ammonium formate kunye ne-formate dehydrogenase).
➢ Udidi ngalunye lwe-ES-PDH oluhambelana neemeko ezahlukeneyo zokusabela, ezinokuphononongwa ngokwahlukeneyo xa kuyimfuneko.
➢ I-ES-PDH kufuneka yongezwe okokugqibela kwisistim yokusabela ukugcina umsebenzi.
Umzekelo 1(I-Biosynthesis yephenylalanine derivatives esuka kwiphenylpyruvate derivatives)(1):
Gcina iminyaka emi-2 ngaphantsi -20 ℃.
Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.
1. UFrancesca Paradisi, uStuart Collins, et al.Ijenali yeBiotechnology, 2007, 128, 408-411.
