I-Nitrilase (NIT)
Inkcazelo emfutshane:
Malunga neNitrilase
I-ES-NITs: Ukuguqula ngokuthe ngqo iqela le-cyanide kwiqela le-carboxyl, ukulungiselela i-carboxylic acid, esetyenziswa kakhulu kumayeza, ukukhuselwa kwendalo, ishishini lemichiza, ukongezwa kokutya, i-textile, imveliso yezolimo kunye nezinye iinkalo.
Kukho iintlobo ze-40 zeemveliso ze-Nitrilase (NIT) (Inombolo njenge-ES-NIT-101~ES-NIT-140) ephuhliswe yi-SyncoZymes.I-ES-NIT sisixhobo esiluncedo ekwenzeni i-chiral carboxylic acids ngokusebenzisa i-regio- kunye ne-stereoselective hydrolysis yoluhlu olubanzi lwe-aliphatic kunye ne-aromatics nitriles.
Uhlobo lwempendulo yeCatalytic:
Iselula/Wechat/WhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | ES-NIT-101~ ES-NIT-140 | iseti ye-40 Nitrilase, 50 mg nganye 40 izinto * 50mg / into, okanye enye ubungakanani |
| Ikhithi yokuHlola (SynKit) | ES-NIT-4000 | isethi 40 Nitrilase, 1 mg nganye 40 izinto * 1mg / into |
★ Ubucacileyo besubstrate ephezulu.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.
➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer (i-optimum reaction pH) kunye ne-ES-NIT.
➢ Zonke ii-ES-NITs zinokuvavanywa ngokulandelelanayo kwi-reaction system engentla okanye nge-NIT Screening Kit (SynKit NIT).
➢ Zonke iintlobo ze-ES-NIT ezingqamene neemeko ezahlukeneyo zokusabela kufuneka zifundwe zodwa.
➢ Uxinzelelo oluphezulu lweSubstrate okanye imveliso enento enokuthi ithintele umsebenzi weES-NIT.Nangona kunjalo, isithintelo sinokukhululeka ngokongezwa kwebhetshi ye-substrate.
Umzekelo 1(Ukwenziwa kwe nicotinic acid)(1):
Umzekelo 2(Uvelwano lwe (R)-(-)-mandelic acid)(2):
Umzekelo 3(Ukwenziwa kwe-baclofen)(3):
Umzekelo 4(Ukwenziwa kwe glycolic acid)(4):
Gcina iminyaka emi-2 ngaphantsi -20 ℃.
Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.
1 Mathew CD, Nagasawa T, Kobayashi M, e tal.Appl.Engqonge.I-Microbiol, i-1988, i-54 (4): i-1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal.Appl.Engqonge.I-Microbiol, i-1991, i-57 (10): i-3028.
3 Xu MZ, Ren J, Gong JS, e tal.Isilevu.J. Biotechnol, 2013, 29(1): 31.
4 Wu S, Fogiel AJ, Petrillo KL, e tal.I-Biotechnol.Bioeng., 2008, 99(3): 717.
