I-Nucloside phosphoryalse (NP)

Inkcazelo emfutshane:

Malunga ne-nucleoside phosphoryalse

Kukho iintlobo ezi-3 zeemveliso ze-enzyme ze-NP (Inombolo njenge-ES-NP-101~ ES-NP-103) ephuhliswe yi-SyncoZymes.I-ES-NP-101 i-purine nucleoside phosphorylase, i-ES-NP-102 kunye ne-ES-NP-103 yi-pyrimidine nucleoside phosphorylase.I-Nucleosidephosphorylase inokubola i-nucleosides kwiziseko kunye ne-pentose phosphate.I-Nucleoside phosphorylase inokwahlulwa ibe yi-purine nucleoside phosphorylase kunye ne-pyrimidine nucleoside phosphorylase ngokokukhethwa kweziseko ze-nucleoside.I-Purine nucleoside phosphorylase inokumetabolise i-adenosine kwi-adenine, inosine kwi-hypoxanthine, i-guanosine kwi-guanine, kwaye ivelise i-ribose phosphate ngexesha elifanayo.I-Pyrimidine nucleoside phosphorylase inokumetabolisa i-uridine kwi-uracil kwaye ivelise i-ribose phosphate.

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyile:lchen@syncozymes.com

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Uhlobo lokusabela kweNucloside phosphoryalse:

Ulwazi ngeMveliso:

Iienzymes	Ikhowudi yeMveliso	Inkcazo
Umgubo we-Enzyme	ES-NP-101~ ES-NP-103	iseti ye-3 Nuclosidephosphoryalse, 50 mg nganye 3 izinto * 50mg / into, okanye enye ubungakanani

Inkqubo yokuhlolwa kweNucloside phosphoryalse:

Le ndlela yokusabela ilandelayo ingasetyenziselwa ireferensi:
I-1-10 mg / ml Substrate
10 mg/ml Enzyme
100 mM isithinteli sePhosphate (pH7.0)
Fuka iintshukumo kwi-shaker yobushushu engaguqukiyo (umzekelo, 30oC, 150rpm) ngeeyure ezingama-12 ~ 24.I-Centrifuge umxube ngamnye ukunyusa iprotheni, i-supernatant ingasetyenziselwa i-TLC, i-HPLC okanye uhlalutyo lwe-GC.