Umfanekiso obonisiweyo weNucloside phosphorylse (NP)

I-Nucloside phosphorylse (NP)

Inkcazo emfutshane:

Malunga ne-nucleoside phosphorylse

Kukho iintlobo ezi-3 zeemveliso ze-enzyme ze-NP (Inombolo njenge-ES-NP-101~ ES-NP-103) eziphuhliswe yiSyncoZymes. I-ES-NP-101 yi-purine nucleoside phosphorylase, i-ES-NP-102 kunye ne-ES-NP-103 yi-pyrimidine nucleoside phosphorylase. I-Nucleosidephosphorylase inokubola ii-nucleosides zibe ziziseko kunye ne-pentose phosphate. I-Nucleoside phosphorylase inokwahlulwa ibe yi-purine nucleoside phosphorylase kunye ne-pyrimidine nucleoside phosphorylase ngokwendlela ezikhethwa ngayo iisiseko ze-nucleoside. I-purine nucleoside phosphorylase inokugaya i-adenosine ibe yi-adenine, i-inosine ibe yi-hypoxanthine, i-guanosine ibe yi-guanine, kwaye ivelise i-ribose phosphate ngaxeshanye. I-Pyrimidine nucleoside phosphorylase inokugaya i-uridine ibe yi-uracil kwaye ivelise i-ribose phosphate.

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I-imeyile:lchen@syncozymes.com

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Iinkcukacha zeMveliso

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Uhlobo lokusabela kwe-Nucloside phosphorylse:

Ulwazi lweMveliso:

Iienzymes	Ikhowudi yeMveliso	Inkcazo
Umgubo we-Enzyme	ES-NP-101~ ES-NP-103	iseti ye-Nuclosidephosphorylse ezi-3, i-50 mg nganye, izinto ezi-3 * 50mg / into, okanye olunye ubungakanani

Inkqubo yokuhlola iimpendulo ze-Nucloside phosphorylse:

Le nkqubo ilandelayo yokusabela ingasetyenziselwa ukubhekisa:
I-substrate eyi-1-10 mg/ml
I-Enzyme eyi-10 mg/ml
I-100 mM Phosphate buffer (pH7.0)
Faka ii-reactions kwi-temperature shaker engaguqukiyo (umzekelo, i-30oC, i-150rpm) iiyure ezili-12 ukuya kwezingama-24. Faka i-centrifuge kumxube ngamnye ukuze ulungelelanise iproteni, apho i-supernatant ingasetyenziselwa uhlalutyo lwe-TLC, i-HPLC okanye i-GC.