I-Transaminase (ATA)
Iienzyme: Zii-macromolecular biological catalysts, uninzi lwee-enzyme ziiproteni.
Ii-Transaminases: Ludidi lwee-enzymes ezikhuthaza ukudluliselwa kwee-amino phakathi kwee-amino acids kunye nee-keto acids. Ii-Transaminases zii-enzymes ezibalulekileyo zebhayoloji kwi-asymmetric synthesis kunye ne-racemic resolution ye-chiral amines.
I-Aminotransferase inokwahlulwahlulwa ibe ziiklasi ezine ngokolandelelwano kunye nolwakhiwo: Ⅰ, Ⅱ, Ⅲ kunye Ⅳ. Ii-ω-aminotransferase zeze-transaminases zeklasi yesiBini, ezisetyenziswa kakhulu ekulungiseleleni ii-chiral amines kunye nee-amino acids ezingezizo ezendalo, ezifana nee-β-amino acids.
Ii-ω-aminotransferases: Kwiimeko ezininzi, i-ω-transaminase ibhekisa kudidi lwee-enzymes, ezithi ii-catalytic ammonia transfer reactions ngaphandle kwe-α-amino acid njenge-substrate okanye imveliso.
Indlela yokwenza i-catalytic:
| Iienzymes | Ikhowudi yeMveliso | Ikhowudi yeMveliso |
| Umgubo we-Enzyme | ES-ATA-101~ ES-ATA-165 | iseti ye-65 ω-Transaminases, 50 mg nganye 65 izinto * 50mg / into, okanye olunye ubungakanani |
| Isixhobo sokuvavanya (iSynKit) | I-ES-ATA-6500 | iseti ye-65 ω-Transaminases, 1 mg nganye Izinto ezingama-65 * 1mg / into |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Ukusebenza kakuhle kokuguqula.
★ Iimveliso ezingaphantsi kwezinye.
★ Iimeko zokusabela okuncinci.
★ Inobuhlobo nokusingqongileyo.
➢ Ukuhlolwa kwee-enzyme kufuneka kwenziwe kwiindawo ezithile ngenxa yobungakanani be-substrate, kwaye kufunyanwe i-enzyme evuselela i-substrate ekujoliswe kuyo enefuthe elihle kakhulu le-catalytic.
➢ Ungaze udibane neemeko ezinzima ezifana nobushushu obuphezulu, i-pH ephezulu/ephantsi kunye nesinyibilikisi sendalo esinoxinzelelo oluphezulu.
➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka iquke i-substrate, isisombululo se-buffer, i-amino donor (ezifana nee-amino acids kunye ne-1-phenyl ethylamine) okanye i-receptor (ezifana nee-keto acids), i-coenzyme (PLP), i-cosolvent (ezifana ne-DMSO).
➢ I-ATA kufuneka yongezwe okokugqibela kwinkqubo yokusabela, emva kokuba i-pH kunye nobushushu zilungisiwe kwimeko yokusabela.
➢ Zonke iintlobo ze-ATA zineemeko ezahlukeneyo zokusabela ezifanelekileyo, ngoko ke nganye yazo kufuneka ifundwe ngakumbi nganye nganye.
Umzekelo 1 (ukwenziwa kweSitagliptin, ukwenziwa okungalinganiyo)(1):
Umzekelo 2 (iMexiletine, Udibaniso lwesisombululo sekinetic kunye nokuhlanganiswa okungalinganiyo)(2):
1 USavile CK, uJaney JM, uMundorff EC, nabanye. iSayensi, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al. Iileta ze-Organic, 2009,11 (21): 4810-4812.
