I-Transaminase (ATA)
Ii-Enzymes: Ngaba zii-macromolecular biological catalysts, uninzi lwee-enzymes ziiproteni.
Transaminases: Iklasi ye-enzymes eyenza ukudluliselwa kwe-amino phakathi kwe-amino acids kunye ne-keto acids.I-Transaminase zii-enzyme eziphambili zebhayoloji kwi-asymmetric synthesis kunye nesisombululo sobuhlanga se-chiral amines.
I-Aminotransferase inokwahlulwa ibe ziiklasi ezine ngokolandelelwano kunye nesakhiwo: Ⅰ, Ⅱ, Ⅲ kunye Ⅳ.I-ω-aminotransferases iyingxenye yeklasi ye-II transaminases, esetyenziswa ngokuqhelekileyo ekulungiseleleni i-chiral amines kunye ne-amino acids engekho ngokwemvelo, njenge-β-amino acids.
I-ω-aminotransferases: Kwiimeko ezininzi, i-ω-transaminase ibhekisela kwiklasi ye-enzymes, ukuba i-catalytic ammonia yokudlulisa iimpendulo ngaphandle kwe-α-amino acid njenge-substrate okanye imveliso.
Indlela yokwenza:
| Iienzymes | Ikhowudi yeMveliso | Ikhowudi yeMveliso |
| Umgubo we-Enzyme | ES-ATA-101~ ES-ATA-165 | iseti ye-65 ω-Transaminases, 50 mg nganye 65 izinto * 50mg / into, okanye enye ubungakanani |
| Ikhithi yokuHlola (SynKit) | ES-ATA-6500 | iseti ye-65 ω-Transaminases, 1 mg nganye 65 izinto * 1mg / into |
★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ uguqulo olusebenzayo.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.
➢ Ukuhlolwa kwe-Enzyme kufuneka kuqhutywe kwi-substrates ezithile ngenxa ye-substrate ekhethekileyo, kwaye ufumane i-enzyme eyenza i-substrate ekujoliswe kuyo kunye neyona nto isebenzayo.
➢ Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye ne-organic solvent ene-concentration ephezulu.
➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer, i-amino donor (efana ne-amino acids kunye ne-1-phenyl ethylamine) okanye i-receptor (njenge-keto acids), i-coenzyme (PLP), i-cosolvent (njenge-DMSO).
➢ I-ATA kufuneka yongezwe ekugqibeleni kwinkqubo yokuphendula, emva kokuba i-pH kunye nobushushu bulungelelaniswe kwimeko yokusabela.
➢ Zonke iintlobo ze-ATA zineemeko ezahlukeneyo zokusabela, ngoko ke nganye kuzo kufuneka iqhutyelwe phambili ngokufunda ngayo.
Umzekelo 1(i-synthesis ye-Sitagliptin, i-asymmetric synthesis)(1):
Umzekelo 2 (iMexiletine, Udibaniso lwesisombululo sekinetic kunye ne-asymmetric synthesis)(2):
1 Savile CK, Janey JM, Mundorff EC, et al.Inzululwazi, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al.Iileta ze-Organic, 2009,11 (21): 4810-4812.
