I-Alcohol oxidase (AOX)
Inkcazelo emfutshane:
Malunga ne-alcohol oxidase
I-ES-AOXs: Ezi enzymes ziyakwazi ukwenza i-oxidation yee-alcohols ezinamafutha okanye i-aryl-alcohols ukwenza i-aldehydes.Basebenzisa ioksijini yemolekyuli kodwa ayifuni icofactor eyongeziweyo ngaphandle, kunye nehydrogen peroxide iveliswa.
Kukho iintlobo ezi-6 zeemveliso ze-alcohol oxidases (Inani njenge-ES-AOX-101~ES-AOX-106) ephuhliswe yi-SyncoZymes.I-ES-AOX101 kunye ne-ES-AOX102 zikhetha i-aliphatic alcohol substrates, i-ES-AOX103~ ES-AOX105 ikhetha i-alcohol substrates enuka kamnandi, kwaye i-ES-AOX106 yi-cholesterol oxidase.I-SZ-AOX sisixhobo esiluncedo sokwenza i-oxidation yee-alcohols ezinamafutha okanye i-aryl-alcohols ukuvelisa i-aldehydes.
Uhlobo lwe-Catalytic reaction:
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I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | ES-AOX-101~ ES-AOX-105 | iseti ye-5 alcohol oxidases, 50 mg nganye 5 izinto * 50mg / into, okanye obunye ubungakanani |
| Ikhithi yokuHlola (SynKit) | ES-AOX-500 | iseti ye-5 alcohol oxidases, 50 mg nganye 5 izinto * 50mg / into, okanye obunye ubungakanani |
★ Ubucacileyo besubstrate ephezulu.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.
➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer kunye ne-ES-AOX, kunye ne-oxygen kufuneka ibe khona.
➢ Zonke iintlobo ze-ES-AOXs ezingqamana neemeko ezahlukeneyo zokusabela, ezinokuphononongwa ngabanye.
➢ Uxinzelelo oluphezulu lweSubstrate okanye imveliso enento enokuthintela ukusebenza kweES-AOX.Nangona kunjalo, isithintelo sinokukhululeka ngokongezwa kwebhetshi ye-substrate.
➢ Ingqokelela ye-H2O2kwinkqubo iya kubangela ukungasebenzi kwe-enzyme, enokuthi ixazululwe ngokufanelekileyo ngokusetyenziswa kwe-catalase.
Umzekelo 1(I-Oxidation ye-aryl-alcohols)(1):
Umzekelo 2(I-Oxidation yeealkoholi ezinamafutha)(2):
Gcina iminyaka emi-2 ngaphantsi -20 ℃.
Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.
1. Benen J AE, Sa' nchez-Torres P, Wagemaker M JM, e tal.J Biol Chem, 1998, 273 (14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, e tal.Appl Microbiol Biot, 1992, 37: 66-73.
