I-oxidase yotywala (AOX)
Inkcazo emfutshane:
Malunga ne-oxidase yotywala
Ii-ES-AOX: Ezi enzymes ziyakwazi ukushukumisa i-oxidation ye-fatty alcohols okanye i-aryl-alcohols ukuze zenze i-aldehydes. Zisebenzisa i-molecule oxygen kodwa azifuni i-cofactor eyongeziweyo ngaphandle, kunye ne-hydrogen peroxide eveliswayo.
Kukho iintlobo ezi-6 zeemveliso ze-alcohol oxidases (Inombolo njenge-ES-AOX-101~ES-AOX-106) eziphuhliswe yiSyncoZymes. I-ES-AOX101 kunye ne-ES-AOX102 zikhetha ii-substrates ze-aliphatic alcohol, i-ES-AOX103~ ES-AOX105 zikhetha ii-substrates ze-alcohol ezinuka kamnandi, kwaye i-ES-AOX106 yi-cholesterol oxidase. I-SZ-AOX sisixhobo esiluncedo sokubangela i-oxidation yee-fatty alcohols okanye ii-aryl-alcohols ukuvelisa i-aldehydes.
Uhlobo lwempendulo ye-Catalytic:
Iselfowuni/iWechat/iWhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | ES-AOX-101~ ES-AOX-105 | iseti yee-oxidases ezi-5 zotywala, i-50 mg nganye, izinto ezi-5 * 50mg / into, okanye olunye ubungakanani |
| Isixhobo sokuvavanya (iSynKit) | I-ES-AOX-500 | iseti yee-oxidases ezi-5 zotywala, i-50 mg nganye, izinto ezi-5 * 50mg / into, okanye olunye ubungakanani |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Uguquko oluphezulu.
★ Iimveliso ezingaphantsi kwezinye.
★ Iimeko zokusabela okuncinci.
★ Inobuhlobo nokusingqongileyo.
➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka iquke i-substrate, isisombululo se-buffer kunye ne-ES-AOX, kwaye kufuneka kubekho i-oxygen.
➢ Zonke iintlobo ze-ES-AOXs ezihambelana neemeko ezahlukeneyo zokusabela ezifanelekileyo, ezinokufundwa nganye nganye.
➢ I-substrate enoxinzelelo oluphezulu okanye imveliso enoxinzelelo oluphezulu inokuthintela umsebenzi we-ES-AOX. Nangona kunjalo, uthintelo lunokupheliswa ngokongeza i-substrate ngebhetshi.
➢ Ukuqokelelwa kwe-H2O2kwinkqubo kuya kukhokelela ekungasebenzini kwee-enzyme, nto leyo enokusombulula ngempumelelo ngokusebenzisa i-catalase.
Umzekelo 1 (Ukufakwa kwe-aryl-alcohols kwi-oxidation)(1):
Umzekelo 2 (Ukunyibilika kwe-oxidation ye-fatty alcohols)(2):
Gcina iminyaka emibini ngaphantsi kwe -20℃.
Ungaze udibane neemeko ezinzima ezifana nobushushu obuphezulu, i-pH ephezulu/ephantsi kunye nesinyibilikisi sendalo esinoxinzelelo oluphezulu.
1. Benen J AE, Sa' nchez-Torres P, Wagemaker M JM, e tal. J Biol Chem, 1998, 273 (14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, e tal. Appl Microbiol Biot, 1992, 37: 66-73.
