Esterase&Lipase (PLE&CALB) Umfanekiso okhoyo

I-Esterase&Lipase (PLE&CALB)

Inkcazelo emfutshane:

Malunga ne-Esterase&Lipase

I-ES-PLEs: Iklasi ye-hydrolases eyenza ukubunjwa kunye nokuphulwa kweebhondi ze-ester.Banokuthatha inxaxheba kwiindidi ezininzi ze-biochemical reactions, ezifana ne-esterification, i-transesterification kunye ne-hydrolysis.Zisetyenziswa ngokubanzi kwiinkalo ezininzi ezifana nokulungiswa kokutya, ishishini lemichiza, i-biomedicine, amayeza e-chiral kunye nonyango lokusingqongileyo.

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyile:lchen@syncozymes.com

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Malunga ne-Esterase&Lipase:

Kukho iindidi ezingama-26 zeemveliso ze-enzyme ye-PLE (Inani njenge-ES-PLE-101～ES-PLE-126) ephuhliswe yi-SyncoZymes.I-ES-PLE ingasetyenziselwa i-hydrolysis ye-aliphatic kunye ne-ester compounds, okanye isisombululo se-regioselective kunye ne-stereoselective ukudibanisa i-chiral acids kunye ne-derivatives yazo.

Uhlobo lwe-Catalytic reaction:

Izinto eziluncedo:

★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.

Imiyalelo yokusetyenziswa:

➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer kunye ne-ES-PLE.I-esterification yezinye ii-ES-PLE zenziwa kwisigaba sezinto eziphilayo.
➢ Zonke iintlobo zee-ES-PLE ezingqamene neemeko ezahlukeneyo zokusabela kufuneka zifundwe zodwa.

Imizekelo yosetyenziso:

Umzekelo 1(I-Biosynthesis ye-Pregabalin ePhakathi)⁽¹⁾:

Umzekelo 2⁽²⁾:

Umzekelo 3⁽³⁾:

Umzekelo 4⁽⁴⁾:

Ugcino:

Gcina iminyaka emi-2 ngaphantsi -20 ℃.

Ingqalelo:

Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi.

IiReferensi:

1. Xu FX, Chen SY, Xu G, e tal.Appl.I-Biotechnol Bioproc E, i-1988, i-54 (4): i-1030.
2. Huang, FC, Lee, LF, Mittal, RSD e tal.J. Am.Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal.Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron:Asymmetry 2000, 11, 917

Ngaphambili: I-Epoxide hydrolase (EH)

Okulandelayo: Formate dehydrogenase (FDH)

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