I-Benzaldehyde lyase (BAL)
Inkcazo emfutshane:
Malunga neBenzaldehyde lyase
I-ES-BAL (iBenzaldehyde lyase): uhlobo lwe-enzyme evuselela i-hydroxymethylation ye-aldehydes e-aromatic kunye ne-aliphatic kunye ne-formaldehyde ukuvelisa i-α-Hydroxymethyl ketone ehambelanayo. I-BAL yi-enzyme exhomekeke kwi-thiamine diphosphate (ThDP). Kukho uhlobo olunye kuphela lwemveliso ye-enzyme ye-BAL (Inombolo njenge-ES-BAL101) eyenziwe yiSyncoZymes, enokusetyenziswa kwi-hydroxymethylation ye-aldehydes e-aromatic kunye ne-aliphatic.
Uhlobo lwempendulo ye-Catalytic:
Iselfowuni/iWechat/iWhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Uguquko oluphezulu.
★ Iimveliso ezingaphantsi kwezinye.
★ Iimeko zokusabela okuncinci.
★ Inobuhlobo nokusingqongileyo.
➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka iquke i-substrate, isisombululo se-buffer, i-enzyme, i-ThDP, i-Mg2+.
➢ I-BAL kufuneka yongezwe okokugqibela kwinkqubo yokusabela, emva kokuba i-pH kunye nobushushu zilungisiwe kwimeko yokusabela.
Umzekelo 1 (Ukwenziwa kwee-amino alcohols eziyi-1,2 ezithathelwe indawo yi-N ezivela kwi-aldehydes)(1):
Gcina iminyaka emibini ngaphantsi kwe -20℃.
Ungaze udibane neemeko ezinzima ezifana nobushushu obuphezulu, i-pH ephezulu/ephantsi kunye nesinyibilikisi sendalo esinoxinzelelo oluphezulu.
1. ULi Y, uHu N, uXu Z, nabanye. I-Angewandte Chemie International Edition, 2022, 61(17): e202116344.
