SyncoZymes

iimveliso

I-Epoxide hydrolase (EH)

Inkcazelo emfutshane:

Malunga ne-epoxide hydrolase

I-EH yi-ether hydrolase eyenza i-stereoselective hydrolysis ye-racemic epoxides ukuvelisa i-1, i-2-diol kunye ne-optical active epoxides, engasetyenziselwa ukulungiswa kwe-chiral ye-epoxides okanye ukulungiswa kwe-chiral diols.Zisetyenziswa kakhulu kwezamayeza nakwezinye iindawo.Kukho iintlobo ezi-3 zeemveliso ze-epoxide hydrolases (Inani njenge-ES-EH-101~ES-EH-103) ephuhliswe yi-SyncoZymes.I-ES-EH sisixhobo esiluncedo sokwenza i-synthesis ye-chiral alcohols ngokusebenzisa i-stereoselective hydrolysis ye-aliphatic kunye ne-aromatic epoxides.

Uhlobo lwempendulo yeCatalytic:

I-Epoxide hydrolase EH2

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyile:lchen@syncozymes.com


Iinkcukacha zeMveliso

Iithegi zeMveliso

Ulwazi ngeMveliso:

I-Epoxide hydrolase EH
Iienzymes Ikhowudi yeMveliso Inkcazo
Umgubo we-Enzyme ES-EH-101~ ES-EH-103 iseti ye-3 Epoxide Hydrolases, 50 mg nganye 3 izinto * 50mg / into, okanye enye ubungakanani
Ikhithi yokuHlola (SynKit) ES-EH-300 isethi ye-3 Epoxide Hydrolases, 1 mg nganye izinto ezi-3 * 1mg / into

Izinto eziluncedo:

★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.

Imiyalelo yokusetyenziswa:

➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer (optimum reaction pH) kunye ne-ES-EH.
➢ Zonke iintlobo zee-ES-EHs ezingqamene neemeko ezahlukeneyo zokusabela kufuneka zifundwe zodwa.
➢ Uxinzelelo oluphezulu lweSubstrate okanye imveliso enayo inokuthintela ukusebenza kweES-EH.Nangona kunjalo, isithintelo sinokukhululeka ngokongezwa kwebhetshi ye-substrate.

Imizekelo yosetyenziso:

Umzekelo 1(1):

I-Epoxide hydrolase EH3

Ugcino:

Gcina iminyaka emi-2 ngaphantsi -20 ℃.

Ingqalelo:

Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.

IiReferensi:

1. Kim YH, Lee I, Choi SH, e tal.J Mol Catal B-Enzym, 2013, 89: 48-51.


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