Umfanekiso obonisiweyo weCytochrome P450 monooxygenase (CYP)

I-cytochrome P450 monooxygenase (CYP)

Inkcazo emfutshane:

Malunga ne-CYP

I-ES-CYPs: Ludidi lwee-enzymes ze-redox ezine-mercaptan-heme njengeziko elisebenzayo lokuguqula ukuhanjiswa kweoksijini. Zii-oxygenases zesiphelo kwaye zifuna i-heme ukuze isebenze ioksijini.

I-Cytochrome P450 monooxygenase ivuselela i-oxidation, i-epoxidation kunye ne-hydroxylation ye-alkyl, i-hydroxylation kunye ne-oxidation ye-ammonia, i-oxidation ye-sulfur, i-dealkylation (i-oxygen, i-sulfur, i-ammonia), i-oxidative dehydrogenation, i-deamination kunye ne-dehalogenation.
Kukho iintlobo ezisi-8 zeemveliso ze-cytochrome P450 monooxygenase (Inombolo njenge-ES-CYP-101～ES-CYP-108) ezenziwe yiSyncoZymes.

Iselfowuni/iWechat/iWhatsApp: +86-13681683526

I-imeyile:lchen@syncozymes.com

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Malunga ne-CYP:

Uhlobo lwempendulo ye-Catalytic:

Ulwazi lweMveliso:

Iienzymes	Isixhobo sokuvavanya (iSynKit)	Inkcazo
Umgubo we-Enzyme	I-ES-CYP-101~ I-ES-CYP-108	iseti yee-8 Cytochrome P450 Monooxygenases, 50 mg nganye 8 izinto * 50mg / into, okanye olunye ubungakanani
Isixhobo sokuvavanya (iSynKit)	I-ES-CYP-800	iseti yee-8 Cytochrome P450 Monooxygenases, 1mg nganye Izinto ezi-8 * 1mg / into

Iingenelo:

★ Uluhlu olubanzi lwe-substrate.
★ Uguquko oluphezulu.
★ Iimveliso ezingaphantsi kwezinye.
★ Iimeko zokusabela okuncinci.
★ Inobuhlobo nokusingqongileyo.

Imiyalelo yokusebenzisa:

➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka iquke i-substrate, isisombululo se-buffer (I-pH yempendulo efanelekileyo ye-enzyme), i-coenzyme (i-NAD(H) okanye i-NADP(H)), inkqubo yokuvuselela i-coenzyme (umz. i-glucose kunye ne-glucose dehydrogenase) kunye ne-ES-CYP. inkqubo yokuvuselela i-coenzyme kunye ne-coenzyme inokutshintshwa yi-hydrogen peroxide kwinkqubo yokusabela engaphelelanga.
➢ Zonke iintlobo ze-ES-CYPs ezihambelana neemeko ezahlukeneyo zokusabela ezifanelekileyo kufuneka zifundwe nganye nganye.
➢ I-substrate enoxinzelelo oluphezulu okanye imveliso enayo inokuthintela umsebenzi we-ES-CYP. Nangona kunjalo, uthintelo lunokupheliswa ngokongeza i-substrate ngebhetshi.

Imizekelo yoSetyenziso:

Umzekelo 1⁽¹⁾:

Umzekelo 2⁽²⁾:

Umzekelo 3⁽³⁾:

Umzekelo 4⁽⁴⁾:

Indawo yokugcina:

Gcina iminyaka emibini ngaphantsi kwe -20℃.

Ingqwalasela:

Ungaze udibane neemeko ezinzima ezifana nobushushu obuphezulu, i-pH ephezulu/ephantsi kunye nesinyibilikisi sendalo esinoxinzelelo oluphezulu.

Iireferensi:

1. UZaretzki J, uMatlock M, kunye noSwamidass S JJ Chem. Imodeli yolwazi, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez F A., e tal. J. Am. Ikhem. Soc., 2006, 128 (26), 8374-8375.
3. Cryle M J., Matovic N J., kunye noDe Voss J J. Org. Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal. Biochim. I-Biophys. Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.; Cheon, WK e tal. Acta Crystallog. 2009, 65, 372.