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I-SyncoZymes

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Umfanekiso obonisiweyo we-Ene reductase (ERED)
  • I-Ene reductase (ERED)

I-Ene reductase (ERED)

Inkcazo emfutshane:

Malunga ne-ene reductase

Ii-ES-ERED: Ziyinxalenye yosapho lwee-enzymes ezindala ezityheli ezinciphisa ngokukhetha i-C=C kwiikhompawundi ze-α, β-unsaturated ezinamaqela afunxa ii-electron. Zisetyenziswa kakhulu kuphononongo lwee-amino acid derivatives, izinto eziphilayo, izinto ezidityanisiweyo zamayeza, iziqholo kunye neebhloko ze-chiral.

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I-imeyile:lchen@syncozymes.com


Iinkcukacha zeMveliso

Iithegi zeMveliso

Malunga ne-ene reductase:

Ii-ES-ERED zivuselela iintlobo ezahlukeneyo ze-substrate ngenxa yeentlobo ezahlukeneyo ze-substrate. Ngokubanzi, i-C=C yee-α, ii-β-unsaturated compounds ezinamaqela afunxa ii-electron (kuquka i-ketone aldehyde, amaqela e-nitro, ii-carboxylic acids, ii-esters, i-anhydride, ii-lactones, ii-imines, njl.njl.) zincitshiswa lula yi-ES-EREDs, kodwa ii-uncated double bonds azincitshiswa.

Kukho iintlobo ezingama-46 zeemveliso ze-enzyme ze-ERED (Inombolo njenge-ES-ERED-101~ES-ERED-146) eziphuhliswe yiSyncoZymes.

Indlela yokwenza i-catalytic:

I-Ene reductase ERED1

Ulwazi lweMveliso:

I-Ene reductase ERED2
Iienzymes Ikhowudi yeMveliso Inkcazo
Umgubo we-Enzyme ES-ERED-101~ ES-ERED-146 iseti ye-46 Ene Reductases, 50 mg nganye, izinto ezingama-46 * 50mg / into, okanye olunye ubungakanani
Isixhobo sokuvavanya (iSynKit) ES-ERED-4600 iseti ye-46 Ene Reductases, 50 mg nganye, izinto ezingama-46 * 50mg / into, okanye olunye ubungakanani

Iingenelo:

★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Uguquko oluphezulu.
★ Iimveliso ezingaphantsi kwezinye.
★ Iimeko zokusabela okuncinci.
★ Inobuhlobo nokusingqongileyo.
★ Ukhuseleko oluphezulu.

Imiyalelo yokusebenzisa:

➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka iquke i-substrate, isisombululo se-buffer (i-pH yempendulo efanelekileyo), ii-coenzymes (i-NAD(H) okanye i-NADP(H)), inkqubo yokuvuselela i-coenzyme (umz. i-glucose kunye ne-glucose dehydrogenase) kunye ne-ES-ERED.
➢ Zonke ii-ES-ERED zingavavanywa ngokulandelelana kwinkqubo yokusabela engentla okanye nge-ERED Screening Kit (SynKit ERED).
➢ Zonke iintlobo ze-ES-EREDs ezihambelana neemeko ezahlukeneyo zokusabela ezifanelekileyo kufuneka zifundwe nganye nganye.
➢ I-substrate enoxinzelelo oluphezulu okanye imveliso enayo inokuthintela umsebenzi we-ES-ERED. Nangona kunjalo, uthintelo lunokupheliswa ngokongeza i-substrate ngebhetshi.

Imizekelo yoSetyenziso:

Umzekelo 1(α,β-unsaturated aldehydes okanye iiketones)(1):

I-Ene reductase ERED3

Umzekelo 2(α,β-unsaturated carboxylic acids kunye neziphumo zazo)(2):

I-Ene reductase ERED4

Indawo yokugcina:

Gcina iminyaka emibini ngaphantsi kwe -20℃.

Ingqwalasela:

Ungaze udibane neemeko ezinzima ezifana nobushushu obuphezulu, i-pH ephezulu/ephantsi kunye nesinyibilikisi sendalo esinoxinzelelo oluphezulu.

Iireferensi:

1. Lucídio C, Fardelone J, Augusto R, e tal. J.Mol.Catal.B:Enzym., 2004, 29: 41-45.
2. UStueckler C, Hall M, Ehammer H, etal. .Org.Lett, 2007, 9(26): 5409-5411.

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