I-Imine reductase (IRED)
Inkcazo emfutshane:
Malunga ne-Imine reductase
I-ES-IRED: Ludidi lwee-enzymes ezikhuthaza ukunciphisa i-C=N bond kwi-CN bond. Yeye-redox enzyme, ene-coenzyme NADPH yokuthutha i-hydrogen. Ngokwendlela ezithandwa ngayo iimveliso ze-chiral, zinokwahlulwa zibe yi-R-IRED kunye ne-S-IRED. I-Syncozymes yenze i-15 imine reductases (numbIRED ES-IRED-101-ES-IRED-115), ezingasetyenziswa kakhulu ekunciphiseni i-regioselective kunye ne-stereoselective ye-imines ukulungiselela i-chiral amines.
Uhlobo lwempendulo ye-Catalytic:
Iselfowuni/iWechat/iWhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | ES-IRED-101~ ES-IRED-114 | iseti ye-14 Imine Reductase, 50 mg nganye |
| Ikhithi yokuvavanya ii-enzyme ze-96-Well | I-ES-IRED-1400 | iseti ye-14 Imine Reductase, 1 mg nganye |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Uguquko oluphezulu.
★ Iimveliso ezingaphantsi kwezinye.
★ Iimeko zokusabela okuncinci.
★ Inobuhlobo nokusingqongileyo.
➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka iquke i-substrate, isisombululo se-buffer, i-enzyme, i-coenzyme, kunye nenkqubo yokuvuselela i-coenzyme.
➢ Zonke iintlobo ze-ES-IREDs ezihambelana neemeko ezahlukeneyo zokusabela ezifanelekileyo kufuneka zifundwe nganye nganye.
➢ I-substrate enoxinzelelo oluphezulu okanye imveliso enayo inokuthintela umsebenzi we-ES-IRED. Nangona kunjalo, uthintelo lunokupheliswa ngokongeza i-substrate ngebhetshi.
Umzekelo 1 (I-Biosynthesis ye-chiral 2-methyl pyrrolidine)(1):
Umzekelo 2 (i-Biosynthesis ye-amine yesibini)(2):
Umzekelo 3 (Ukunciphisa iiCyclic Imines)(3):
Gcina iminyaka emibini ngaphantsi kwe -20℃.
Ungaze udibane neemeko ezinzima ezifana nobushushu obuphezulu, i-pH ephezulu/ephantsi kunye nesinyibilikisi sendalo esinoxinzelelo oluphezulu.
1. Scheller PN, Fademrecht S, Hofelzer S, et al. I-ChemBioChem, i-2014, i-15, i-2201-2204.
2. Wetzl D, Gand M, Ross A, et al. ChemCatChem, 2016, 8, 2023-2026.
3. ULi H, uLuan ZJ, uZheng GW, et al. Adv. Synth. I-Catal. 2015, 357, 1692-1696.
