I-Imine reductase (IRED)
Inkcazelo emfutshane:
Malunga noImine reductase
I-ES-IRED: Iklasi ye-enzymes eyenza ukunciphisa i-C = N ibhondi kwi-CN bond.Yeye-enzyme ye-redox, kunye ne-coenzyme NADPH yokuthutha i-hydrogen.Ngokokhetho lwe-chiral lweemveliso, zinokwahlulwa zibe yi-R-IRED kunye ne-S-IRED.I-syncozymes iphuhliswe i-15 imine reductases (i-numbIRED ES-IRED-101-ES-IRED-115), enokusetyenziswa ngokubanzi ekunciphiseni i-regioselective kunye ne-stereoelective ye-imines ukulungiselela i-chiral amines.
Uhlobo lwe-Catalytic reaction:
Iselula/Wechat/WhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | ES-IRED-101~ ES-IRED-114 | iseti ye-14 Imine Reductase, i-50 mg nganye |
| 96-Well Enzyme Screening Kit | ES-IRED-1400 | isethi ye-14 Imine Reductase, i-1 mg nganye |
★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.
➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer, i-enzyme, i-coenzyme, kunye nenkqubo yokuvuselela i-coenzyme.
➢ Zonke iintlobo ze-ES-IRED ezingqamene neemeko ezahlukeneyo zokusabela kufuneka zifundwe zodwa.
➢ Uxinzelelo oluphezulu lweSubstrate okanye imveliso enayo inokuthintela ukusebenza kwe-ES-IRED.Nangona kunjalo, isithintelo sinokukhululeka ngokongezwa kwebhetshi ye-substrate.
Umzekelo 1(I-Biosynthesis ye-chiral 2-methyl pyrrolidine)(1):
Umzekelo 2(I-Biosynthesis ye-amine yesibini)(2):
Umzekelo 3 (Ukuncitshiswa kwe-Imines ye-Cyclic)(3):
Gcina iminyaka emi-2 ngaphantsi -20 ℃.
Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.
1. Scheller PN, Fademrecht S, Hofelzer S, et al.I-ChemBioChem, i-2014, i-15, i-2201-2204.
2. Wetzl D, Gand M, Ross A, et al.ChemCatChem, 2016, 8, 2023-2026.
3. U-Li H, uLuan ZJ, uZheng GW, et al.Adv.Synth.I-Catal.2015, 357, 1692-1696.
