Umfanekiso obonisiweyo we-CALB engasebenziyo

I-CALB engasebenziyo

Inkcazo emfutshane:

I-CALB

I-Recombinant Lipase B evela kwiCandida antarctica (CALB) iveliswa ngokubiliswa okungaphantsi komhlaba ngePichia pastoris eguqulwe ngokwezakhi zofuzo.

I-CALB ingasetyenziswa kwi-water phase okanye kwi-organic phase catalytic esterification, i-esterosis, i-transesterification, i-ring opening polyester synthesis, i-aminolysis, i-hydrolysis ye-amides, i-acylation ye-amines kunye ne-addition reaction.

I-CALB inokukhetha okuphezulu kwe-chiral kunye nokukhetha isikhundla, ngoko ke ingasetyenziswa kakhulu kwimveliso yeoyile, ukutya, amayeza, izinto zokuthambisa kunye nezinye iikhemikhali.

Iselfowuni/iWechat/iWhatsApp: +86-13681683526

I-imeyile:lchen@syncozymes.com

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

I-CALB engasebenziyo:

I-CALB ayishukumi ngenxa yokufunxwa ngokwasemzimbeni kwi-resin ethanda amanzi amaninzi, eyi-polymer enezimbobo ezinkulu, i-styrene/methacrylate. I-CALB engashukumiyo ifanelekile ukusetyenziswa kwizinyibilikisi zendalo nakwiinkqubo ezingenazo izinyibilikisi, kwaye ingaphinda isetyenziswe kwaye isetyenziswe amaxesha amaninzi kwiimeko ezifanelekileyo.
Ikhowudi yeMveliso: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.

Iingenelo ze-SZ-CALB-IMMO100:

★Umsebenzi ophezulu, ukukhetha okuphezulu kwe-chiral kunye nozinzo oluphezulu.
★Ukusebenza ngcono kwizigaba ezingezizo ezomanzi.
★Susa ngokulula kwinkqubo yokusabela, uphelise ngokukhawuleza ii-reaction, kwaye uphephe intsalela yeproteni kwimveliso.
★Ingaphinda isetyenziswe kwaye isetyenziswe kwakhona ukunciphisa iindleko.

Iiparameter zeMveliso ze-SZ-CALB-IMMO100:

Umsebenzi	≥10000PLU/g
uluhlu lwe-pH yokusabela	5-9
Uluhlu lobushushu bokusabela	10-60℃
Inkangeleko	I-CALB-IMMO100-A: Iqinile elimthubi okhanyayo ukuya kumdaka I-CALB-IMMO100-B: I-solid emhlophe ukuya kwemdaka kancinci
Ubungakanani beenxalenye	300-500μm
Ilahleko xa yomiswa kwi-105℃	0.5%-3.0%
I-resin yokuthintela ukungasebenzi	I-polymer ene-macroporous, i-styrene/methacrylate
Isinyibilikisi sempendulo	Amanzi, isinyibilikisi sendalo, njl.njl., okanye ngaphandle kwesinyibilikisi. Kwimpendulo kwezinye izinyibilikisi zendalo, i-3% yamanzi ingongezwa ukuphucula isiphumo sempendulo
Ubungakanani beenxalenye	I-CALB-IMMO100-A: 200-800 μm I-CALB-IMMO100-B: 400-1200 μm

Inkcazo yeyunithi: Iyunithi e-1 ihambelana nokuhlanganiswa kwe-1μmol ngomzuzu we-propyl laurate evela kwi-lauric acid kunye ne-1-propanol kwi-60℃. I-CALB-IMMP100-A kunye ne-CALB-IMMO100-B ezingentla zihambelana nabathwali abangakwaziyo ukuhamba abanobukhulu obahlukeneyo bezinto ezincinci.

Imiyalelo kunye namanyathelo okuzikhusela:

1. Uhlobo lwe-reactor
I-enzyme engashukumiyo isebenza kwi-kettle batch reactor nakwi-fixed bed continuous flow reactor. Kufuneka kuqatshelwe ukuba kuthintelwe ukutyumza ngenxa yamandla angaphandle ngexesha lokutya okanye ukuzalisa.
2. I-pH yempendulo, ubushushu kunye nesinyibilikisi
I-enzyme engasebenziyo kufuneka yongezwe ekugqibeleni, emva kokuba ezinye izinto zongeziwe kwaye zinyibilikiswe, kwaye i-pH ilungiswe.
Ukuba ukusetyenziswa kwe-substrate okanye ukwakheka kwemveliso kuya kukhokelela ekutshintsheni kwe-pH ngexesha lokusabela, kufuneka kongezwe i-buffer eyaneleyo kwinkqubo yokusabela, okanye i-pH kufuneka ijongwe kwaye ilungiswe ngexesha lokusabela.
Ngaphakathi koluhlu lokunyamezela ubushushu lwe-CALB (ngaphantsi kwama-60 ℃), izinga lokuguqulwa liye landa ngokunyuka kobushushu. Xa kusetyenziswa ngokwenene, ubushushu bokusabela kufuneka bukhethwe ngokuhambelana nokuzinza kwesiseko okanye imveliso.
Ngokubanzi, i-ester hydrolysis reaction ifanelekile kwinkqubo yesigaba samanzi, ngelixa i-ester synthesis reaction ifanelekile kwinkqubo yesigaba sendalo. I-organic solvent ingaba yi-ethanol, i-tetrahydrofuran, i-n-hexane, i-n-heptane kunye ne-toluene, okanye i-mixed solvent efanelekileyo. Kwi-reaction kwezinye izinyibilikisi zendalo, i-3% yamanzi ingongezwa ukuphucula isiphumo se-reaction.
3. Ukusetyenziswa kwakhona kunye nexesha lokusebenza kwe-CALB
Phantsi kwemeko efanelekileyo yokusabela, i-CALB inokufunyanwa kwakhona kwaye isetyenziswe kwakhona, kwaye amaxesha athile okufaka isicelo ayahluka ngokweeprojekthi ezahlukeneyo.
Ukuba i-CALB efunyenweyo ayisetyenziswa rhoqo kwaye kufuneka igcinwe emva kokubuyiselwa, kufuneka ihlanjwe yomiswe kwaye ivalwe kwi-2-8 ℃.
Emva kokusetyenziswa kwakhona izihlandlo ezininzi, ukuba ukusebenza kakuhle kwempendulo kuncitshisiwe kancinci, i-CALB ingongezwa ngokufanelekileyo kwaye iqhubeke nokusetyenziswa. Ukuba ukusebenza kakuhle kwempendulo kuncitshisiwe kakhulu, kufuneka itshintshwe.

Imizekelo yoSetyenziso:

Umzekelo 1 (i-Aminolysis)⁽¹⁾:

Umzekelo 2 (i-Aminolysis)⁽²⁾:

Umzekelo 3 (Ukwenziwa kwe-polyester evula iringi)⁽³⁾:

Umzekelo 4 (Ukuguqulwa kwee-ester, ukukhetha kwakhona iqela le-hydroxyl)⁽⁴⁾:

Umzekelo 5 (Ukuguqulwa kwee-ester, isisombululo se-kinetic se-racemic alcohols)⁽⁵⁾:

Umzekelo 6 (Ukuqinisekiswa, isisombululo se-kinetic se-carboxylic acid)⁽⁶⁾:

Umzekelo 7 (i-Esterolysis, isisombululo se-kinetic)⁽⁷⁾:

Umzekelo 8 (iHydrolysis ye-amides)⁽⁸⁾:

Umzekelo 9 (Ukunyuka kwe-amines)⁽⁹⁾:

Umzekelo 10 (impendulo yokongezwa kuka-Aza-Michael)⁽¹⁰⁾:

Iireferensi:

1. Chen S, Liu F, Zhang K, e tal. Tetrahedron Lett, 2016, 57: 5312-5314.
2. Olah M, Boros Z, anszky GH, e tal. Tetrahedron, 2016, 72: 7249-7255.
3. Nakaoki1 T, Mei Y, Miller LM, etal. Ind. Biotechnol, 2005, 1(2):126-134.
4. Pawar SV, Yadav G DJ Ind. Eng. Chem, 2015, 31: 335-342.
5. I-Kamble MP, iShinde SD, iYadav G DJ Mol. Catal. B: I-Enzym, 2016, 132: 61-66.
6. Shinde SD, Yadav G D. Inkqubo yeBiochem, 2015, 50: 230-236.
7. Souza TC, Fonseca TS, Costa JA, e tal. J. Mol. I-Catal. B: Enzym, 2016, 130: 58-69.
8. Gavi´an AT, Castillo E, L´opez-Mungu´AJ Mol. Catal. B: Enzym, 2006, 41: 136-140.
9. Joubioux FL, Henda YB, Bridiau N, e tal. J. Mol. I-Catal. B: Enzym, 2013, 85-86: 193-199.
10. Dhake KP, Tambade PJ, Singhal RS, e tal. Tetrahedron Lett, 2010, 51: 4455-4458.