Umfanekiso obonisiweyo we-Nitrile reductase (NRED)

I-Nitrile reductase (NRED)

Inkcazo emfutshane:

Malunga ne-Nitrile reductase

Kukho iintlobo ezintathu zemveliso ye-enzyme ye-NRED (Inombolo njenge-ES-NRED101~ ES-NRED103) eyenziwe yiSyncoZymes. I-NRED inokwenza i-catalyst inciphise iqela le-nitrile ibe yi-amine. Kwinkqubo ye-catalysis, i-NADPH iyadingeka njengomthuthi we-hydrogen.

Uhlobo lokusabela lwe-Nitrile reductasecatalytic

I-Nitrile reductase NRED2

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I-imeyile:lchen@syncozymes.com

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ulwazi lweMveliso:

Iienzymes	Ikhowudi yeMveliso	Inkcazo
Inkcazo	ES- NRED -101~ ES- NRED -103	iseti ye-3 Nitrile Reductase, 50 mg nganye Izinto ezi-3 * 50mg / into, okanye olunye ubungakanani

Inkqubo yokuhlola iimpendulo ze-Nitrile reductase:

Le nkqubo ilandelayo yokusabela ingasetyenziselwa ukubhekisa:
I-substrate eyi-1-10 mg/ml
I-Enzyme eyi-10 mg/ml
10% (v/v) I-organic cosolvent (1)
I-100 mM Phosphate buffer (pH7.0)
0.2 mg/ml NADP+
I-10 mg/ml yeglucose
2 mg/ml I-glucose dehydrogenase (GDH)
Faka ii-reactions kwi-temperature shaker engaguqukiyo (umzekelo, i-30oC, i-150rpm) iiyure ezingama-24 ukuya kuma-48. Faka i-centrifuge kumxube ngamnye ukuze ulungelelanise iproteni, apho i-supernatant ingasetyenziselwa uhlalutyo lwe-TLC, HPLC okanye i-GC.
Qaphela (1): Isinyibilikisi sendalo (esifana ne-DMSO, i-methanol, i-acetonitrile, i-IPA, njl.njl.) asinyanzelekanga, sisetyenziselwa izinto ezinganyibilikiyo.