I-Nitro reductase (NTR)
Uhlobo lwe-Catalytic reaction:
Indlela yokwenza:
Iienzymes | Ikhowudi yeMveliso | Inkcazo |
Umgubo we-Enzyme | ES-NTR-101~ ES-NTR-112 | iseti ye-12 ye-Nitro Reductases, i-50 mg nganye kwizinto ze-12 * 50mg / into, okanye enye ubungakanani |
Ikhithi yokuHlola (SynKit) | ES-NTR-1200 | iseti ye-12 ye-Nitro Reductases, i-1 mg nganye yezinto ezili-12 * 1mg / into |
★ I-spectrum ebanzi yesubstrate.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.
➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer (optimum reaction pH), i-coenzymes (NAD (H) okanye i-NADP (H)), inkqubo yokuvuselela i-coenzyme (umzekelo, i-glucose kunye ne-glucose dehydrogenase) kunye ne-ES-NTR.
➢ Zonke iintlobo ze-ES-NTR ezingqamana neemeko ezahlukeneyo zokusabela kufuneka zifundwe zodwa.
➢ I-ES-NTR kufuneka yongezwe okokugqibela kwisixokelelwano sokusabela nge-pH kunye nobushushu obuphezulu.
Umzekelo 1(1):
Umzekelo 2(2):
Gcina iminyaka emi-2 ngaphantsi -20 ℃.
Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.
1 Dai RJ, Chen J,Lin J, e tal.J Hazard Marer, 2009, 170, 141-143.
2 Betancor L, Berne C, Luckarift H R., e tal.Ikhem.Commun, 2006, 3640-3642.