Phenylalanine dehydrogenase (PDH)
Iienzymes | Ikhowudi yeMveliso | Inkcazo |
Umgubo we-Enzyme | ES-PDH-101~ ES-PDH-108 | iseti ye-8 Nitrile Reductase, 50 mg nganye 8 izinto * 50mg / into, okanye enye ubungakanani |
★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.
➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer, i-enzyme, i-coenzyme (i-NAD (H)), inkqubo yokuvuselela i-coenzyme (umz. i-ammonium formate kunye ne-formate dehydrogenase).
➢ Udidi ngalunye lwe-ES-PDH oluhambelana neemeko ezahlukeneyo zokusabela, ezinokuphononongwa ngokwahlukeneyo xa kuyimfuneko.
➢ I-ES-PDH kufuneka yongezwe okokugqibela kwisistim yokusabela ukugcina umsebenzi.
Umzekelo 1(I-Biosynthesis yephenylalanine derivatives esuka kwiphenylpyruvate derivatives)(1):
Gcina iminyaka emi-2 ngaphantsi -20 ℃.
Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.
1. UFrancesca Paradisi, uStuart Collins, et al.Ijenali yeBiotechnology, 2007, 128, 408-411.