SyncoZymes

iimveliso

Phenylalanine dehydrogenase (PDH)

Inkcazelo emfutshane:

Malunga nePhenylalanine dehydrogenase

ES-PDH: Iklasi ye-oxidoreductase.Ukusabela okufanelekileyo kunokubangela i-oxidative deamination ye-L phenylalanine ityuwa phambi kwe-NAD ukuvelisa ityuwa ye-phenylpyruvate, kunye ne-reverse reaction can catalyze synthesis ye-amino acid ityuwa ehambelana netyuwa ye-phenylpyruvate.I-Syncozymes iphuhlise izinto ze-8 ze-phenylalanine dehydrogenase (inombolo ye-ES-PDH-101~ES-PDH-108).I-ES-PDH ingasetyenziselwa ukulungiselela ityuwa ye-phenylalanine okanye ityuwa ye-amino acid ehambelanayo esuka kwityuwa ye-phenylpyruvic okanye i-derivatives.
Uhlobo lwe-Catalytic reaction:

IPDH2

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyile:lchen@syncozymes.com


Iinkcukacha zeMveliso

Iithegi zeMveliso

Ulwazi ngeMveliso:

PDH
Iienzymes Ikhowudi yeMveliso Inkcazo
Umgubo we-Enzyme ES-PDH-101~ ES-PDH-108 iseti ye-8 Nitrile Reductase, 50 mg nganye 8 izinto * 50mg / into, okanye enye ubungakanani

Izinto eziluncedo:

★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.

Imiyalelo yokusetyenziswa:

➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer, i-enzyme, i-coenzyme (i-NAD (H)), inkqubo yokuvuselela i-coenzyme (umz. i-ammonium formate kunye ne-formate dehydrogenase).
➢ Udidi ngalunye lwe-ES-PDH oluhambelana neemeko ezahlukeneyo zokusabela, ezinokuphononongwa ngokwahlukeneyo xa kuyimfuneko.
➢ I-ES-PDH kufuneka yongezwe okokugqibela kwisistim yokusabela ukugcina umsebenzi.

Imizekelo yosetyenziso:

Umzekelo 1(I-Biosynthesis yephenylalanine derivatives esuka kwiphenylpyruvate derivatives)(1):

IPDH3

Ugcino:

Gcina iminyaka emi-2 ngaphantsi -20 ℃.

Ingqalelo:

Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.

IiReferensi:

1. UFrancesca Paradisi, uStuart Collins, et al.Ijenali yeBiotechnology, 2007, 128, 408-411.


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