I-Amine Dehydrogenase (AmDH)
Inkcazo emfutshane:
Malunga ne-Amine Dehydrogenase
I-ES-AmDH (i-Amine Dehydrogenase): i-enzyme enokushukumisa i-ammoniation yeqela le-carbonyl ize iyinciphise ibe liqela le-amino. Yi-enzyme exhomekeke kwi-coenzyme NADH, kwaye i-NADH isebenza njengomthumeli we-hydrogen. Kukho iintlobo ezine zeemveliso ze-enzyme ze-AmDH (Inombolo njenge-ES-AmDH101 ~ ES-AmDH104) eyenziwe yiSyncoZymes, engasetyenziswa kwi-regioselective okanye stereoselective reductive amination ye-aldehydes kunye neeketones.
Uhlobo lwempendulo ye-Catalytic:
Iselfowuni/iWechat/iWhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Uguquko oluphezulu.
★ Iimveliso ezingaphantsi kwezinye.
★ Iimeko zokusabela okuncinci.
★ Inobuhlobo nokusingqongileyo.
➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka iquke i-substrate, isisombululo se-buffer, i-enzyme, i-coenzyme kunye nenkqubo yokuvuselela i-coenzyme (umz. i-glucose kunye ne-glucose dehydrogenase).
➢ I-AmDH kufuneka yongezwe okokugqibela kwinkqubo yokusabela, emva kokuba i-pH kunye nobushushu zilungisiwe kwimeko yokusabela.
Umzekelo 1 (Ukwenziwa kwee-chiral amines ezihambelanayo ngokunciphisa i-alkyl aryl ketones)(1):
Gcina iminyaka emibini ngaphantsi kwe -20℃.
Ungaze udibane neemeko ezinzima ezifana nobushushu obuphezulu, i-pH ephezulu/ephantsi kunye nesinyibilikisi sendalo esinoxinzelelo oluphezulu.
1. Kong W, Liu Y, Huang C, et al. I-Angewandte Chemie International Edition, 2022: e202202264.
