I-Cyclohexanone monooxygenase (CHMO)
Iienzymes | Ikhowudi yeMveliso | Inkcazo |
Ikhithi yokuHlola (SynKit) | ES-CHMO-101 | isethi ye-1 Ketoreductases, 1 mg nganye 1 izinto * 1mg / into |
★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.
➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer, i-enzyme, i-coenzyme kunye nenkqubo yokuvuselela i-coenzyme (umz. i-glucose kunye ne-glucose dehydrogenase).
➢ I-CHMO kufuneka yongezwe okokugqibela kwisistim yokusabela, emva kokuba i-pH kunye nobushushu bulungelelaniswe kwimeko yokusabela.
Umzekelo 1(I-Synthesis ye-cyclohexanol esuka kwi-cyclohexanol)(1):
Gcina iminyaka emi-2 ngaphantsi -20 ℃.
Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.
1. Friso, Marco, Appl Microbiol Biotechnol, 2017, 101 (20): 7557-7565