Umfanekiso obonisiweyo we-Epoxide hydrolase (EH)

I-Epoxide hydrolase (EH)

Inkcazo emfutshane:

Malunga ne-epoxide hydrolase

I-EH yi-ether hydrolase evuselela i-stereoselective hydrolysis yee-racemic epoxides ukuvelisa i-1, 2-diol kunye nee-epoxides ezisebenzayo, ezinokusetyenziselwa isisombululo se-chiral seepoxides okanye ukulungiswa kwee-chiral diols. Zisetyenziswa kakhulu kwezonyango nakwamanye amasimi. Kukho iintlobo ezi-3 zeemveliso ze-epoxide hydrolases (Inombolo njenge-ES-EH-101～ES-EH-103) eziphuhliswe yiSyncoZymes. I-ES-EH sisixhobo esiluncedo sokuvuselela ukwenziwa kwee-chiral alcohols ngokusebenzisa i-stereoselective hydrolysis yee-aliphatic kunye nee-aromatic epoxides.

Uhlobo lwempendulo ye-Catalytic:

I-Epoxide hydrolase EH2

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I-imeyile:lchen@syncozymes.com

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ulwazi lweMveliso:

Iienzymes	Ikhowudi yeMveliso	Inkcazo
Umgubo we-Enzyme	ES-EH-101~ ES-EH-103	iseti ye-Epoxide Hydrolases ezi-3, i-50 mg nganye, izinto ezi-3 * 50mg / into, okanye olunye ubungakanani
Isixhobo sokuvavanya (iSynKit)	ES-EH-300	iseti ye-Epoxide Hydrolases ezi-3, i-1 mg nganye Izinto ezi-3 * 1mg / into

Iingenelo:

★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Uguquko oluphezulu.
★ Iimveliso ezingaphantsi kwezinye.
★ Iimeko zokusabela okuncinci.
★ Inobuhlobo nokusingqongileyo.

Imiyalelo yokusebenzisa:

➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka iquke i-substrate, isisombululo se-buffer (i-pH yempendulo efanelekileyo) kunye ne-ES-EH.
➢ Zonke iintlobo ze-ES-EHs ezihambelana neemeko ezahlukeneyo zokusabela ezifanelekileyo kufuneka zifundwe nganye nganye.
➢ I-substrate enoxinzelelo oluphezulu okanye imveliso enayo inokuthintela umsebenzi we-ES-EH. Nangona kunjalo, uthintelo lunokupheliswa ngokongeza i-substrate ngebhetshi.