I-Epoxide hydrolase (EH)
Iienzymes | Ikhowudi yeMveliso | Inkcazo |
Umgubo we-Enzyme | ES-EH-101~ ES-EH-103 | iseti ye-3 Epoxide Hydrolases, 50 mg nganye 3 izinto * 50mg / into, okanye enye ubungakanani |
Ikhithi yokuHlola (SynKit) | ES-EH-300 | isethi ye-3 Epoxide Hydrolases, 1 mg nganye izinto ezi-3 * 1mg / into |
★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.
➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer (optimum reaction pH) kunye ne-ES-EH.
➢ Zonke iintlobo zee-ES-EHs ezingqamene neemeko ezahlukeneyo zokusabela kufuneka zifundwe zodwa.
➢ Uxinzelelo oluphezulu lweSubstrate okanye imveliso enayo inokuthintela ukusebenza kweES-EH.Nangona kunjalo, isithintelo sinokukhululeka ngokongezwa kwebhetshi ye-substrate.
Umzekelo 1(1):
Gcina iminyaka emi-2 ngaphantsi -20 ℃.
Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.
1. Kim YH, Lee I, Choi SH, e tal.J Mol Catal B-Enzym, 2013, 89: 48-51.