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Umfanekiso obonisiweyo we-Esterase kunye neLipase (PLE kunye neCALB)
  • I-Esterase kunye neLipase (PLE kunye neCALB)
  • I-Esterase kunye neLipase (PLE kunye neCALB)

I-Esterase kunye neLipase (PLE kunye neCALB)

Inkcazo emfutshane:

Malunga ne-Esterase kunye neLipase

Ii-ES-PLE: Udidi lwee-hydrolases ezikhuthaza ukwakheka nokuqhekeka kwee-ester bonds. Zingathatha inxaxheba kwiintlobo ezininzi zeempendulo ze-biochemical, ezifana ne-esterification, i-transesterification kunye ne-hydrolysis. Zisetyenziswa kakhulu kwiindawo ezininzi ezifana nokulungiswa kokutya, ishishini leekhemikhali, i-biomedicine, amayeza e-chiral kunye nonyango lokusingqongileyo.

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I-imeyile:lchen@syncozymes.com


Iinkcukacha zeMveliso

Iithegi zeMveliso

Malunga ne-Esterase kunye neLipase:

Kukho iintlobo ezingama-26 zeemveliso ze-enzyme ze-PLE (Inombolo njenge-ES-PLE-101～ES-PLE-126) eziphuhliswe yiSyncoZymes. I-ES-PLE ingasetyenziselwa i-hydrolysis yeekhompawundi ze-aliphatic kunye ne-ester, okanye isisombululo se-regioselective kunye ne-stereoselective ukwenza ii-chiral acids kunye ne-derivatives zazo.

Uhlobo lwempendulo ye-Catalytic:

I-Esterase kunye neLipase (PLE kunye neCALB)
I-Esterase kunye neLipase (PLE kunye neCALB)1

Iingenelo:

★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Uguquko oluphezulu.
★ Iimveliso ezingaphantsi kwezinye.
★ Iimeko zokusabela okuncinci.
★ Inobuhlobo nokusingqongileyo.

Imiyalelo yokusebenzisa:

➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka iquke i-substrate, isisombululo se-buffer kunye ne-ES-PLE. Ukuqinisekiswa kwezinye ii-ES-PLE kwenziwa kwisigaba se-organic.
➢ Zonke iintlobo ze-ES-PLEs ezihambelana neemeko ezahlukeneyo zokusabela ezifanelekileyo kufuneka zifundwe nganye nganye.

Imizekelo yoSetyenziso:

Umzekelo 1 (iBiosynthesis yePregabalin Intermediate)(1):

i-calb1

Umzekelo 2(2):

i-calb2

Umzekelo 3(3):

i-calb3

Umzekelo 4(4):

i-calb5

Indawo yokugcina:

Gcina iminyaka emibini ngaphantsi kwe -20℃.

Ingqwalasela:

Ungaze udibane neemeko ezinzima ezifana nobushushu obuphezulu, i-pH ephezulu/ephantsi.

Iireferensi:

1. Xu FX, Chen SY, Xu G, etal. Appl. Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD e tal. J. Am. Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal. Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron:Asymmetry 2000, 11, 917

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