I-Monoamine oxidase (MAO)
Iienzymes | Ikhowudi yeMveliso | Inkcazo |
Umgubo we-Enzyme | ES-MAO-101~ ES-MAO-105 | iseti ye-5 Monoamine Oxidase, 50 mg nganye 5 izinto * 50mg / into, okanye enye ubungakanani |
Ikhithi yokuHlola (SynKit) | ES-MAO-500 | iseti ye-5 ye-Monoamine Oxidase, i-1 mg nganye yezinto ezi-5 * 1mg / into |
★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela kancinci..
★ Ilungele indalo.
➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer, i-enzyme, i-coenzyme, kunye nenkqubo yokuvuselela i-coenzyme.
➢ Zonke iintlobo ze-MAO ezihambelana neemeko ezahlukeneyo zokusabela kufuneka zifundwe nganye nganye.
➢ Uxinzelelo oluphezulu lweSubstrate okanye imveliso enento enokuthintela umsebenzi weES-MAO.Nangona kunjalo, isithintelo sinokukhululeka ngokongezwa kwebhetshi ye-substrate.
Umzekelo 1(Biosynthesis of (1R,5S)-6,6-dimethyl-3-aza-bicyclo[3.1.0]hex-2-ene)(1):
Gcina iminyaka emi-2 ngaphantsi -20 ℃.
Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.
1 Tao Li, Jack Liang, Alexandre Ambrogelly et al.J. Am.Ikhem.Soc.2012, 134, 6467−6472.