I-Nitrilase (i-NIT)
Inkcazo emfutshane:
Malunga neNitrilase
Ii-ES-NIT: Guqula iqela le-cyanide ngqo libe liqela le-carboxyl, ukulungiselela i-carboxylic acid, esetyenziswa kakhulu kwezonyango, ukhuseleko lokusingqongileyo, kushishino lweekhemikhali, kwizithako zokutya, kwimpahla yelaphu, kwimveliso yezolimo nakwezinye iindawo.
Kukho iintlobo ezingama-40 zeemveliso zeNitrilase (NIT) (Inombolo njenge-ES-NIT-101~ES-NIT-140) eziphuhliswe yiSyncoZymes. I-ES-NIT sisixhobo esiluncedo sokuvuselela ukwenziwa kwee-chiral carboxylic acids ngokusebenzisa i-regio- kunye ne-stereoselective hydrolysis yoluhlu olubanzi lwee-aliphatic kunye nee-aromatic nitriles.
Uhlobo lwempendulo ye-Catalytic:
Iselfowuni/iWechat/iWhatsApp: +86-13681683526
I-imeyile:lchen@syncozymes.com
| Iienzymes | Ikhowudi yeMveliso | Inkcazo |
| Umgubo we-Enzyme | I-ES-NIT-101~ I-ES-NIT-140 | iseti ye-40 Nitrilase, 50 mg nganye Izinto ezingama-40 * 50mg / into, okanye olunye ubungakanani |
| Isixhobo sokuvavanya (iSynKit) | I-ES-NIT-4000 | iseti ye-40 Nitrilase, 1 mg nganye Izinto ezingama-40 * 1mg / into |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Uguquko oluphezulu.
★ Iimveliso ezingaphantsi kwezinye.
★ Iimeko zokusabela okuncinci.
★ Inobuhlobo nokusingqongileyo.
➢ Ngokwesiqhelo, inkqubo yokusabela kufuneka iquke i-substrate, isisombululo se-buffer (i-pH yempendulo efanelekileyo) kunye ne-ES-NIT.
➢ Zonke ii-ES-NIT zingavavanywa ngokulandelelana kwinkqubo yokusabela engentla okanye nge-NIT Screening Kit (SynKit NIT).
➢ Zonke iintlobo ze-ES-NITs ezihambelana neemeko ezahlukeneyo zokusabela ezifanelekileyo kufuneka zifundwe nganye nganye.
➢ I-substrate enoxinzelelo oluphezulu okanye imveliso enoxinzelelo oluphezulu inokuthintela umsebenzi we-ES-NIT. Nangona kunjalo, uthintelo lunokupheliswa ngokongeza i-substrate ngebhetshi.
Umzekelo 1 (Ukwenziwa kwe-nicotinic acid)(1):
Umzekelo 2 (Ukwenziwa kwe-(R)-(-)-mandelic acid)(2):
Umzekelo 3 (Ukwenziwa kwe-baclofen)(3):
Umzekelo 4 (Ukwenziwa kwe-glycolic acid)(4):
Gcina iminyaka emibini ngaphantsi kwe -20℃.
Ungaze udibane neemeko ezinzima ezifana nobushushu obuphezulu, i-pH ephezulu/ephantsi kunye nesinyibilikisi sendalo esinoxinzelelo oluphezulu.
1 Mathew CD, Nagasawa T, Kobayashi M, etal. Appl. Environ. Microbiol, 1988, 54(4): 1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal. Appl. Indalo engqongileyo. I-Microbiol, i-1991, i-57 (10): i-3028.
3 Xu MZ, Ren J, Gong JS, e tal. Isilevu. J. Biotechnol, 2013, 29(1): 31.
4 Wu S, Fogiel AJ, Petrillo KL, e tal. Biotechnol. Bioeng., 2008, 99(3): 717.
