Phenylalanine ammonia lyase (PAL)
Iienzymes | Ikhowudi yeMveliso | Inkcazo |
Umgubo we-Enzyme | ES-PAL-101~ ES-PAL-110 | iseti ye-10 L-phenylalanine ammonia-lyase, 50 mg nganye 10 izinto * 50mg / into, okanye enye ubungakanani |
★ Ubucacileyo besubstrate ephezulu.
★ Ukukhetha okuqinileyo kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Iimveliso ezingaphantsi kweemveliso.
★ Iimeko zokusabela ezithambileyo.
★ Ilungele indalo.
➢ Ngokuqhelekileyo, inkqubo yokusabela kufuneka ibandakanye i-substrate, isisombululo se-buffer kunye ne-enzyme.
➢ Udidi ngalunye lwe-ES-PAL oluhambelana neemeko ezahlukeneyo zokusabela, ezinokuphononongwa ngokwahlukeneyo.
➢ I-ES-PAL kufuneka yongezwe okokugqibela kwisistim yokusabela ukugcina umsebenzi.
Umzekelo 1(I-Biosynthesis ye-trans cinnamic acid esuka kwi-phenylalanine)(1):
Umzekelo 2(I-Biosynthesis yephenylalanine derivative from trans-cinnamic acid derivative)(2):
Gcina iminyaka emi-2 ngaphantsi -20 ℃.
Ungaze udibane neemeko ezigqithisileyo ezinje: ubushushu obuphezulu, i-pH ephezulu/ephantsi kunye noxinzelelo oluphezulu lwe-organic solvent.
1. Zachary Mays, Karishma Mohan,okqhubekayo.I-Chem Commun (Camb).2020, 56, 5255-5258.
2. U-Ian Rowles, uBas Groenendaal,okqhubekayo. iTetrahedron,2016. 72, 7343-7347.